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Updated: Dec 19, 2025
12:04
Examination of Mitotic and Meiotic Fission Yeast Nuclear Dynamics by Fluorescence Live-cell Microscopy
Published on: June 24, 2019
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Selective Nuclear Pore Complex Removal Drives Nuclear Envelope Division in Fission Yeast
María Expósito-Serrano1, Ana Sánchez-Molina1, Paola Gallardo1
1Centro Andaluz de Biología del Desarrollo, Universidad Pablo de Olavide-Consejo Superior de Investigaciones Científicas-Junta de Andalucía, Carretera de Utrera, km1, 41013 Seville, Spain.
Current Biology : CB
|June 6, 2020
Summary
The central spindle guides nuclear division by concentrating and removing nuclear pore complexes (NPCs) at the nuclear envelope. This process, crucial for cell division, fails without importin α.
Area of Science:
- Cell Biology
- Molecular Biology
- Genetics
Background:
- Cell division requires precise organelle partitioning.
- Closed mitosis involves intranuclear spindle elongation for nuclear division.
- Mechanisms of nuclear envelope fission and division site determination are unclear.
Purpose of the Study:
- Investigate the role of the central spindle in nuclear division.
- Elucidate the mechanism of nuclear envelope fission.
- Identify factors regulating nuclear pore complex behavior during division.
Main Methods:
- Utilized fission yeast as a model organism.
- Investigated the function of Ase1/PRC1 in spindle midzone organization.
- Analyzed nuclear pore complex (NPC) localization and dynamics.
- Examined nuclear envelope (NE) remodeling during anaphase B.
- Assessed the impact of importin α absence on nuclear division.
Main Results:
- Ase1/PRC1 at the spindle midzone concentrates nuclear pore complexes (NPCs) at the nuclear envelope (NE).
- NPC components are sequentially eliminated during spindle elongation (anaphase B).
- This elimination is coupled with local NE remodeling, leading to NPC removal and nuclear division.
- In importin α-deficient cells, NPCs remain stable, NE remodeling is absent, and nuclear division fails.
Conclusions:
- The central spindle acts as a spatial cue for nuclear division site determination.
- NPC removal is identified as a key triggering event for nuclear division.
- Importin α is essential for NPC removal and subsequent NE remodeling, facilitating nuclear division.
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