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Related Concept Videos

Photoluminescence: Applications01:14

Photoluminescence: Applications

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Photoluminescence offers a wide range of applications due to its inherent sensitivity and selectivity. This technique allows for both direct and indirect analyses of the analyte. Direct quantitative analysis is possible when the analyte exhibits a favorable quantum yield for fluorescence or phosphorescence. However, an indirect analysis may be feasible if the analyte is not fluorescent or phosphorescent, or if the quantum yield is unfavorable. Indirect methods include reacting the analyte with...
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Synthesis of Core-shell Lanthanide-doped Upconversion Nanocrystals for Cellular Applications
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808 nm-activable core@multishell upconverting nanoparticles with enhanced stability for efficient photodynamic

Raquel Martínez1,2, Ester Polo1,2, Silvia Barbosa2,3

  • 1Centro Singular de Investigación en Química Biolóxica e Materiais Moleculares (CiQUS), Universidade de Santiago de Compostela, 15782, Santiago, Spain.

Journal of Nanobiotechnology
|June 7, 2020
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Summary

This study presents novel upconverting nanoparticles activated by 808 nm light, overcoming limitations of 980 nm excitation for biomedical applications. These stable nanoplatforms enable efficient photodynamic therapy within cells.

Keywords:
Click chemistryPhotodynamic therapyPhotosensitizersPolymer coatingUpconverting nanoparticles

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Area of Science:

  • Nanotechnology
  • Biomedical Engineering
  • Photochemistry

Background:

  • Rare-earth-doped nanoparticles offer unique upconversion properties for biomedical uses like imaging and therapy.
  • Current systems often use 980 nm excitation, which is absorbed by water, causing heating and limiting biological applications.
  • Alternative excitation sources (808 nm, 1064 nm) are needed for robust nanoplatforms in biological settings.

Purpose of the Study:

  • To develop stable, aqueous upconverting nanoparticles activated by 808 nm near-infrared (NIR) light.
  • To create a versatile photodynamic nanoplatform by functionalizing nanoparticles with photosensitizers.
  • To evaluate the efficacy of these nanoplatforms for photodynamic therapy in cellular environments.

Main Methods:

  • Synthesized aqueous-stable upconverting nanoparticles.
  • Functionalized nanoparticles with an amphiphilic polymer using click chemistry.
  • Attached FDA-approved photosensitizers (Rose Bengal, Chlorin e6) to the polymer.
  • Tested nanoparticle stability, photoluminescence, and reactive oxidative species (ROS) generation in biological media and cells.

Main Results:

  • Developed upconverting nanoparticles efficiently activated by 808 nm light, avoiding water absorption issues.
  • Achieved long-term colloidal stability in biological media with retained photoluminescence.
  • Created an 808 nm-activable photodynamic nanoplatform using photosensitizer-conjugated polymers.
  • Demonstrated ROS generation and impact on mitochondrial integrity upon cellular uptake and 808 nm excitation.

Conclusions:

  • Successfully demonstrated 808 nm-activable upconverting nanoplatforms for photodynamic therapy.
  • Nanoplatforms remain photoactive after cellular internalization, enabling targeted ROS generation.
  • The versatile polymer-stabilization strategy allows for future modifications, creating multifunctional nanoreactors for in vivo applications.