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Updated: Dec 19, 2025

Author Spotlight: Optimizing Affinity Chromatography for His-Tagged FEN1 Protein

Author Spotlight: Optimizing Affinity Chromatography for His-Tagged FEN1 Protein

Published on: April 26, 2024

Affinity Tags for Protein Purification.

Vibhor Mishra^1,2

¹Department of Biology, Indiana University, Bloomington, IN 47405, USA

Current Protein & Peptide Science

|June 7, 2020

Summary

Affinity tags are crucial for recombinant protein purification and solubility enhancement. This review details common tags, their classifications (epitope and protein/domain), and removal strategies, aiding researchers in protein biochemistry.

Keywords:

Proteins affinity tag chromatography protease purification solubility

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Protein Complex Affinity Capture from Cryomilled Mammalian Cells

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Orthogonal Protein Purification Facilitated by a Small Bispecific Affinity Tag

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Published on: January 16, 2012

Area of Science:

Biochemistry
Molecular Biology
Protein Engineering

Background:

Affinity tags are essential tools in recombinant protein production.
They facilitate purification and can enhance solubility of difficult proteins.
Combinatorial approaches with affinity tags are effective for purifying protein complexes.

Purpose of the Study:

To review the key features of commonly used affinity tags.
To classify affinity tags into distinct categories.
To discuss strategies for affinity tag removal post-purification.

Main Methods:

Literature review of common affinity tags.
Classification of affinity tags into epitope and protein/domain types.
Discussion of protease-based tag removal techniques.

Main Results:

Affinity tags are N- or C-terminal additions for purification.
Tags can act as solubility enhancers for challenging targets.
Epitope tags bind chromatography resins, while protein/domain tags offer dual functions.

Conclusions:

Carefully selected affinity tags are vital for efficient protein purification.
Understanding tag classifications aids in experimental design.
Protease-based removal is a key consideration after purification.