Batch chromatography with recycle lag. I-Concept and design

Area of Science:

• Chemical Engineering
• Separation Science
• Chromatography

Background:

• Multicolumn simulated countercurrent chromatography (CCC) is widely used for purification.
• Existing CCC methods can be complex and require significant infrastructure.
• There is a need for simpler, scalable chromatographic separation techniques.

Purpose of the Study:

• To explore and extend the theory of batch chromatography with recycle lag.
• To develop a mathematical framework for single-column batch analogues of multicolumn CCC.
• To design, construct, and validate a physical prototype for this concept.

Main Methods:

• Revisiting and extending the theory of batch chromatography with recycle lag.
• Developing mathematical formulations for single-column batch CCC analogues.
• Designing and constructing an eluate recycling device (ERD).
• Experimental validation of the ERD prototype.

Main Results:

• The theory of batch chromatography with recycle lag was mathematically formulated and extended.
• A single-column batch analogue framework was derived for various multicolumn CCC processes.
• A functional eluate recycling device (ERD) was designed, built, and experimentally validated.
• The ERD demonstrated an approximate "first in, first out" eluate recycling method.

Conclusions:

• Batch chromatography with recycle lag offers a simplified approach to chromatographic separations.
• The developed ERD is a practical and scalable solution for implementing this concept.
• This method has potential for large-scale industrial purification applications.