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Related Concept Videos

High-Performance Liquid Chromatography: Elution Process01:05

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In High-Performance Liquid Chromatography (HPLC), the elution process is critical to the separation of analytes and the quality of chromatographic results. Elution describes how compounds move through the column and separate based on their interactions with the mobile and stationary phases. This process determines the resolution, peak shape, and retention times in the chromatogram, which are essential for identifying and quantifying components in complex mixtures. Understanding the elution...
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Principles Of Column Chromatography01:13

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The chromatography technique was first invented in 1901 by Michael S. Tswett, a Russian botanist, to separate plant pigments using organic solvents. Further, in 1941, Archer John Porter Martin and R. L. M. Synge modified the technique by packing silica gel into a column. A mixture of amino acids was then separated on the packed column using chloroform and water mixture as the mobile phase. This was the first report on column chromatography. At present, column chromatography is a widely used...
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Chromatography: Introduction01:10

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Chromatography is a technique used to separate compounds based on differences of partitioning between two phases, the stationary phase and the mobile phase.
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High-performance liquid chromatography, or HPLC, is an analytical technique that separates liquid samples under high pressures. An HPLC instrument consists of glass bottles for storing solvents called mobile phase reservoirs. HPLC-grade solvents are used to maintain high purity, and the dissolved gases are removed using a degasser, such as a vacuum pumping system or sparging with helium. The solvents are then pumped into the analytical column using a screw-driven syringe or reciprocating pumps.
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Chromatographic Methods: Terminology01:18

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Chromatography is an analytical technique widely used in fields such as chemistry, biology, environmental science, and pharmaceuticals to separate the components of a mixture and identify substances between them. The process of chromatography is based on the interactions between two distinct phases: the stationary phase and the mobile phase. The stationary phase is fixed in place by a supporting material, while the mobile phase moves over it, carrying the solutes. As the mobile phase travels,...
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High-Performance Liquid Chromatography: Introduction01:11

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High-performance liquid chromatography(HPLC), formerly referred to as High-pressure liquid chromatography, is a powerful technique used to separate, identify, and quantify components in complex mixtures. The term "high pressure" refers to using high pressure to push the liquid mobile phase through the tightly packed columns.
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Batch chromatography with recycle lag. I-Concept and design.

Abimaelle S Chibério1, Tiago P Santos1, Rui P P L Ribeiro1

  • 1LAQV, REQUIMTE, Department of Chemistry, NOVA School of Science and Technology, Universidade NOVA de Lisboa, Caparica 2829-516, Portugal.

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|June 8, 2020
PubMed
Summary

This study introduces batch chromatography with recycle lag, a novel method for separation processes. A prototype eluate recycling device (ERD) was designed and validated for large-scale applications.

Keywords:
Batch chromatography with recycle lagOne-column analogue of multicolumn chromatographyRecycle chromatography

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Area of Science:

  • Chemical Engineering
  • Separation Science
  • Chromatography

Background:

  • Multicolumn simulated countercurrent chromatography (CCC) is widely used for purification.
  • Existing CCC methods can be complex and require significant infrastructure.
  • There is a need for simpler, scalable chromatographic separation techniques.

Purpose of the Study:

  • To explore and extend the theory of batch chromatography with recycle lag.
  • To develop a mathematical framework for single-column batch analogues of multicolumn CCC.
  • To design, construct, and validate a physical prototype for this concept.

Main Methods:

  • Revisiting and extending the theory of batch chromatography with recycle lag.
  • Developing mathematical formulations for single-column batch CCC analogues.
  • Designing and constructing an eluate recycling device (ERD).
  • Experimental validation of the ERD prototype.

Main Results:

  • The theory of batch chromatography with recycle lag was mathematically formulated and extended.
  • A single-column batch analogue framework was derived for various multicolumn CCC processes.
  • A functional eluate recycling device (ERD) was designed, built, and experimentally validated.
  • The ERD demonstrated an approximate "first in, first out" eluate recycling method.

Conclusions:

  • Batch chromatography with recycle lag offers a simplified approach to chromatographic separations.
  • The developed ERD is a practical and scalable solution for implementing this concept.
  • This method has potential for large-scale industrial purification applications.