Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Electron Microscope Tomography and Single-particle Reconstruction01:07

Electron Microscope Tomography and Single-particle Reconstruction

2.7K
Transmission electron microscopy (TEM) can be used to determine the 3D structure of biological samples with the help of techniques such as electron microscope tomography and single-particle reconstruction. While single-particle reconstruction can examine macromolecules and macromolecular complexes in vitro conditions only, tomography permits the study of cell components or small cells in vivo.
Electron Tomography
Electron tomography can be performed either in TEM or STEM (scanning transmission...
2.7K
Atomic Force Microscopy01:08

Atomic Force Microscopy

4.2K
Atomic force microscopy (AFM) is a type of scanning probe microscopy that can analyze topographic details of various specimens like ceramics, glass, polymers, and biological samples. AFM offers over 1000 times more resolution than the optical imaging system. Images generated from AFM are three-dimensional surface profiles, offering an advantage over the flat, two-dimensional images from other imaging techniques.
The AFM Probe
The probe is regarded as the heart of any AFM setup and comprises the...
4.2K
Atomic Absorption Spectroscopy: Atomization Methods01:25

Atomic Absorption Spectroscopy: Atomization Methods

1.2K
Atomic Absorption Spectroscopy (AAS) atomizes samples through flame atomization or electrothermal atomization. Flame atomization typically involves a nebulizer and spray chamber assembly to combine the sample with a fuel–oxidant mixture, creating a fine aerosol mist that enters a burner. Typically, the fuel and oxidant are combined in an approximately stoichiometric ratio. However, for atoms that are easily oxidized, a fuel-rich mixture may be more advantageous. Only about 5% of the...
1.2K
Overview of Microscopy Techniques01:22

Overview of Microscopy Techniques

14.5K
The early pioneers of microscopy opened a window into the invisible world of microorganisms. In 1830, Joseph Jackson Lister created an essentially modern light microscope. The 20th century saw the development of microscopes that leveraged nonvisible light, such as fluorescence microscopy that uses an ultraviolet light source and electron microscopy that uses short-wavelength electron beams. These advances significantly improved magnification, image resolution, and contrast. By comparison, the...
14.5K
Positron Emission Tomography01:29

Positron Emission Tomography

6.7K
Positron emission tomography (PET) is a medical imaging technique involving radiopharmaceuticals — substances that emit short-lived radiation. Although the first PET scanner was introduced in 1961, it took 15 more years before radiopharmaceuticals were combined with the technique and revolutionized its potential.
One of the main requirements of a PET scan is a positron-emitting radioisotope, which is produced in a cyclotron and then attached to a substance used by the part of the body...
6.7K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Selective expression of light-harvesting complexes alters phospholipid composition in the intracytoplasmic membrane and core complex of purple phototrophic bacteria.

Biochimica et biophysica acta. Bioenergetics·2023
Same author

Circular dichroism and resonance Raman spectroscopies of bacteriochlorophyll b-containing LH1-RC complexes.

Photosynthesis research·2021
Same author

Long non-coding RNA LINC00641 promotes cell growth and migration through modulating miR-378a/ZBTB20 axis in acute myeloid leukemia.

European review for medical and pharmacological sciences·2019
Same author

Phospholipid distributions in purple phototrophic bacteria and LH1-RC core complexes.

Biochimica et biophysica acta. Bioenergetics·2019
Same author

Hierarchical density-based cluster analysis framework for atom probe tomography data.

Ultramicroscopy·2019
Same author

LncRNA625 modulates prostate cancer cells proliferation and apoptosis through regulating the Wnt/β-catenin pathway by targeting miR-432.

European review for medical and pharmacological sciences·2017
Same journal

Efficient methods for wave propagation in electron microscopy.

Ultramicroscopy·2026
Same journal

Unsupervised deep image prior for sparse-view and limited-angle electron tomography.

Ultramicroscopy·2026
Same journal

Determination of the structure of the tertiary phase in the alloy Al<sub>10</sub>Mo<sub>10</sub>Nb<sub>10</sub>Ta<sub>10</sub>Ti<sub>30</sub>Zr<sub>30</sub> using convergent beam electron diffraction.

Ultramicroscopy·2026
Same journal

Predictive drift compensation of multi-frame STEM via live scan modification.

Ultramicroscopy·2026
Same journal

Deep PACBED: Multitask analysis of PACBED images using deep neural networks.

Ultramicroscopy·2026
Same journal

Guided progressive reconstructive imaging: A new quantization-based framework for low-dose, high-throughput and real-time analytical ptychography.

Ultramicroscopy·2026
See all related articles

Related Experiment Video

Updated: Dec 19, 2025

Atom Probe Tomography Analysis of Exsolved Mineral Phases
08:14

Atom Probe Tomography Analysis of Exsolved Mineral Phases

Published on: October 25, 2019

7.6K

Morphological classification of dense objects in atom probe tomography data.

I Ghamarian1, L-J Yu1, E A Marquis1

  • 1Department of Materials Science and Engineering, University of Michigan, Ann Arbor, MI 48109, USA.

Ultramicroscopy
|June 8, 2020
PubMed
Summary
This summary is machine-generated.

Atom probe tomography struggles with quantitative analysis of solute clusters and dislocations. Our new workflow accurately distinguishes these features, improving analysis of structural alloys.

Keywords:
Atom probe tomographyClusteringDislocationQuantification

More Related Videos

From Voxels to Knowledge: A Practical Guide to the Segmentation of Complex Electron Microscopy 3D-Data
12:08

From Voxels to Knowledge: A Practical Guide to the Segmentation of Complex Electron Microscopy 3D-Data

Published on: August 13, 2014

24.9K
Three-Dimensional Particle Shape Analysis Using X-ray Computed Tomography: Experimental Procedure and Analysis Algorithms for Metal Powders
10:10

Three-Dimensional Particle Shape Analysis Using X-ray Computed Tomography: Experimental Procedure and Analysis Algorithms for Metal Powders

Published on: December 4, 2020

2.1K

Related Experiment Videos

Last Updated: Dec 19, 2025

Atom Probe Tomography Analysis of Exsolved Mineral Phases
08:14

Atom Probe Tomography Analysis of Exsolved Mineral Phases

Published on: October 25, 2019

7.6K
From Voxels to Knowledge: A Practical Guide to the Segmentation of Complex Electron Microscopy 3D-Data
12:08

From Voxels to Knowledge: A Practical Guide to the Segmentation of Complex Electron Microscopy 3D-Data

Published on: August 13, 2014

24.9K
Three-Dimensional Particle Shape Analysis Using X-ray Computed Tomography: Experimental Procedure and Analysis Algorithms for Metal Powders
10:10

Three-Dimensional Particle Shape Analysis Using X-ray Computed Tomography: Experimental Procedure and Analysis Algorithms for Metal Powders

Published on: December 4, 2020

2.1K

Area of Science:

  • Materials Science
  • Metallurgy
  • Data Analysis

Background:

  • Atom probe tomography (APT) is crucial for visualizing solute clusters and segregation in alloys.
  • Quantitative analysis of APT data, especially distinguishing solute clusters from decorated dislocations, presents a significant challenge.
  • Existing methods lack robust approaches for differentiating these high-solute-density features.

Purpose of the Study:

  • To develop and validate a computational workflow for accurately distinguishing solute clusters from solute-decorated dislocation lines in atom probe tomography data.
  • To provide a user-friendly tool for quantitative analysis of complex microstructures in structural alloys.
  • To address the limitations in current APT data analysis for materials science applications.

Main Methods:

  • Combined cluster finding, skeleton finder, and morphological classification algorithms.
  • Developed a graphical user interface (GUI) for streamlined workflow implementation.
  • Validated the method on synthetic datasets with known structures and experimental APT data from Alloy 625.

Main Results:

  • Successfully differentiated between solute clusters and solute-decorated dislocation lines in both synthetic and experimental datasets.
  • The workflow demonstrated high accuracy in classifying dense objects based on solute atom distribution.
  • Applied to proton-irradiated Alloy 625, revealing high densities of Si-decorated dislocations and Si-rich clusters.

Conclusions:

  • The developed workflow offers a robust solution for quantitative analysis of APT data, specifically for distinguishing solute clusters and decorated dislocations.
  • The GUI-packaged workflow enhances accessibility and reproducibility in materials characterization.
  • This advancement aids in understanding microstructural evolution and solute behavior in advanced structural alloys.