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Cardiomyocyte Proteome Remodeling due to Isoproterenol-Induced Cardiac Hypertrophy during the Compensated Phase

Ricardo Cambraia Parreira1,2,3, Diana Paola Gómez-Mendoza1, Itamar Couto Guedes de Jesus1

  • 1Departamento de Fisiologia e Biofísica, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Belo Horizonte, Minas Gerais, 31270-901, Brazil.

Proteomics. Clinical Applications
|June 8, 2020
PubMed

Insights

This study reveals molecular changes in early cardiac hypertrophy using proteomic analysis. Identified markers like MYH7 may aid in early diagnosis of heart failure.

Area of Science:

  • Cardiovascular Biology
  • Proteomics
  • Molecular Cardiology

Background:

  • Cardiac hypertrophy is a significant risk factor for heart failure.
  • Early molecular events in compensated cardiac hypertrophy remain incompletely understood.

Purpose of the Study:

  • To comprehensively characterize molecular events in early compensated cardiac hypertrophy at the cardiomyocyte level.
  • To identify potential early diagnostic markers for cardiac hypertrophy.

Main Methods:

  • Quantitative label-free proteomic analysis of cardiomyocytes.
  • Isoproterenol (ISO) treatment in mice to induce hypertrophy.
  • Canonical pathway analysis and confocal microscopy.

Main Results:

  • Isoproterenol-induced hypertrophy activates actin cytoskeleton and integrin-linked kinase (ILK) signaling, while inhibiting sirtuin signaling.
  • Upregulation of myosin-7 (MYH7) impacts cardiac contractile and calcium signaling.
  • Identified potential early markers: APBB1, GOLGA4, HOOK1, KATNA1, KIFBP, MAN2B2, and SLC16A1.

Conclusions:

  • Provides a molecular map of ISO-induced compensated cardiac hypertrophy in cardiomyocytes.
  • Identified marker candidates may facilitate early diagnosis and intervention for cardiac hypertrophy and heart failure.
Abstract

Keywords:
cardiac hypertrophycardiomyocyteisoproterenolmass spectrometryproteomics

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