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Antioxidant capacity of extract from Jiangtang Xiaozhi recipe in vitro
Objective:
To evaluate the antioxidant capacity of the extract from Jiangtang Xiaozhi recipe (JXR) of in vitro.
Methods:
JXR extract was prepared according to previously reported method. In vitro antioxidant assays were used in this experiment, including 1,1-Diphenyl-2-picrylhydrazyl free radical (DPPH) radical scavenging ability, 2-2'-Azinobis-(3-ethylbenzthiazoline-6-sul phonate (ABTS) radical scavenging ability, reducing power assay, fluorescence recovery after photo bleaching assay, β-carotene bleaching assay, ferric thiocyanate assay, and thiobarbituric acid method.
Results:
DPPH, ABTS assay showed that JXR extract had distinct effect on scavenging free radicals; reducing power and ferricreducing-antioxidant power assay showed that JXR extract possessed redox ability; β-Carotene bleaching assay and antioxidant activity in a linoleic acid system using ferric thiocyanate method, thiobarbituric acid assay indicated that JXR extract could effectively inhibit lipid peroxidation, and the effect was better than that of Vitamin C.
Conclusion:
JXR extract has significant antioxidant capacity in vitro.
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