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Published on: May 9, 2018
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Pneumoperitoneum Modifies Serum and Tissue CCL2-CCL5 Expression in Mice
Alfonso Papparella1, Carmine Noviello2, Sara Ranucci1
1Department of Woman, Child, and General and Specialized Surgery, University of Campania "Luigi Vanvitelli," Naples, Italy.
Summary
Carbon dioxide (CO2) pneumoperitoneum during laparoscopy increases RANTES cytokine levels and alters peritoneal CCL5/CCL2 expression, indicating an inflammatory response in rats.
Area of Science:
- Immunology
- Surgical Research
- Cytokine Biology
Background:
- Laparoscopy is a common abdominal surgical technique.
- Carbon dioxide (CO2) pneumoperitoneum is standard for laparoscopic procedures.
- Understanding the physiological effects of CO2 pneumoperitoneum is crucial.
Purpose of the Study:
- To evaluate systemic and morphological peritoneal cytokine changes.
- Specifically, to assess RANTES/CCL5 and MCP-1/CCL2 modifications.
- To investigate these changes in response to CO2 pneumoperitoneum in a rat model.
Main Methods:
- Twenty-five rats were divided into three groups: two experimental (S1, S2) and one control (C).
- CO2 pneumoperitoneum was induced at different pressures (6 and 10 mm Hg) for 30 minutes in experimental groups.
- Serum cytokine levels (RANTES, MCP-1) were measured via ELISA, and peritoneal expression was analyzed immunohistochemically.
Main Results:
- Significant differences in RANTES levels were observed across all groups (P = .0001).
- MCP-1 levels showed no significant difference between groups (P = .159).
- Immunohistochemistry revealed moderate to strong cytoplasmic reactions for CCL5/CCL2 in peritoneal cells of CO2-exposed rats.
Conclusions:
- CO2 pneumoperitoneum induces an inflammatory response.
- This response is characterized by altered plasma RANTES levels.
- Peritoneal expression of CCL5 and CCL2 is also modified by CO2 pneumoperitoneum.
