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Related Concept Videos

Conjugated Proteins02:50

Conjugated Proteins

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Simple proteins and protein complexes contain only amino acids. In contrast, many other proteins, called conjugated proteins, covalently bond with non-protein moieties.
Nucleoproteins are protein complexes that contain nucleic acids, categorized as deoxyribonucleoproteins (DNPs) or ribonucleoproteins (RNPs) respectively. The nucleosome is a typical example of a DNP where nuclear DNA is associated with histone proteins. The major antigen for the Covid-19 virus SARS-CoV is an RNP that is critical...
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Leaky Scanning02:28

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During most eukaryotic translation processes, the small 40S ribosome subunit scans an mRNA from its 5' end until it encounters the first start AUG codon. The large 60S ribosomal subunit then joins the smaller one to initiate protein synthesis. The location of the translation initiation is largely determined by the nucleotides near the start codon as there may be multiple translation initiation sites present on the mRNA.  Marilyn Kozak discovered that the sequence RCCAUGG (where R...
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Related Experiment Video

Updated: Dec 19, 2025

High-throughput Confocal Imaging of Quantum Dot-Conjugated SARS-CoV-2 Spike Trimers to Track Binding and Endocytosis in HEK293T Cells
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Controlling the SARS-CoV-2 Spike Glycoprotein Conformation.

Rory Henderson1,2, Robert J Edwards1,2, Katayoun Mansouri1

  • 1Duke Human Vaccine Institute, Durham NC 27710, USA.

Biorxiv : the Preprint Server for Biology
|June 9, 2020
PubMed
Summary

Scientists analyzed coronavirus spike (S) proteins to understand their mobility. They engineered stable S-protein constructs, offering a new framework for developing effective coronavirus vaccines.

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Area of Science:

  • Virology
  • Structural Biology
  • Vaccine Development

Background:

  • The coronavirus (CoV) spike (S) protein is crucial for viral entry and a key target for vaccines.
  • S-protein flexibility and domain mobility present challenges for immune targeting and vaccine design.

Conclusions:

  • S-protein conformation can be predictably controlled through rational design.
  • Developed a framework for engineering coronavirus S-proteins for improved vaccine applications.
  • Findings pave the way for more effective coronavirus vaccines targeting the S-protein.