Pilot multi-site quality assurance study of batch-mode clinical-scale automated xenon-129 hyperpolarizers

We present a pilot quality assurance (QA) study of spin-exchange optical pumping (SEOP) performed on two nearly identical second-generation (GEN-2) automated batch-mode clinical-scale ¹²⁹Xe hyperpolarizers, each utilizing a convective forced air oven, high-power (~170 W) continuous pump laser irradiation, and xenon-rich gas mixtures (~1.30 atm partial pressure). In one study, the repeatability of SEOP in a 1000 Torr Xe/900 Torr N₂/100 Torr ⁴He (2000 Torr total pressure) gas mixture is evaluated over the course of ~700 gas loading cycles, with negligible decrease in performance during the first ~200 cycles, and with high ¹²⁹Xe polarization levels (avg. %P_Xe = 71.7% with standard deviation σ_PXe = 1.5%), build-up rates (avg. γ_SEOP = 0.019 min^-1 with standard deviation σ_γ = 0.003 min^-1) and polarization lifetimes (avg. T₁ = 90.5 min with standard deviation σ_T = 10.3 min) reported at moderate oven temperature of ~70 °C. Although the SEOP cell in this study exhibited a detectable performance decrease after 400 cycles, the cell continued to produce potentially useable HP ¹²⁹Xe with %P_Xe = 42.3 ± 0.6% even after nearly 700 refill cycles. The possibility of "regenerating" "dormant" (i.e., not used for an extended period of time) SEOP cells using repeated temperature cycling methods to recover %P_Xe is also demonstrated. The quality and consistency of results show significant promise for translation to clinical-scale production of hyperpolarized ¹²⁹Xe contrast agents for imaging and bio-sensing applications.