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Mapping of synovial fibroblast proteins by high resolution two-dimensional polyacrylamide electrophoresis
A Glennås1, A K Thorsrud, H E Rugstad
1Division of Clinical Pharmacology, National Hospital (Rikshospitalet), Oslo, Norway.
Scandinavian Journal of Rheumatology. Supplement
|January 1, 1988
Abstract:
The highly sensitive protein separation technique two-dimensional polyacrylamide gel electrophoresis was used to study the cellular protein synthesis in 1) cultured human rheumatoid and 2) normal synovial fibroblasts and 3) normal skin fibroblasts. No reproducible differences were found that could be ascribed to cellular origin, the disease or in vitro cellular aging. The reasons for that probably were related to the cell cultures and not to failure of protein detection.