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Related Experiment Video

Updated: Dec 19, 2025

Quantitative Optical Microscopy: Measurement of Cellular Biophysical Features with a Standard Optical Microscope
14:09

Quantitative Optical Microscopy: Measurement of Cellular Biophysical Features with a Standard Optical Microscope

Published on: April 7, 2014

16.0K

Molecular height measurement by cell surface optical profilometry (CSOP).

Sungmin Son1, Sho C Takatori1, Brian Belardi1

  • 1Department of Bioengineering and Biophysics Program, University of California, Berkeley, CA 94720.

Proceedings of the National Academy of Sciences of the United States of America
|June 10, 2020
PubMed
Summary
This summary is machine-generated.

Researchers developed cell surface optical profilometry (CSOP) to measure molecular heights on cell surfaces. This method reveals how protein and glycan dimensions impact cell function and interactions.

Keywords:
CD45antibodiescell surface topographyplasma membranesuperresolution microscopy

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Related Experiment Videos

Last Updated: Dec 19, 2025

Quantitative Optical Microscopy: Measurement of Cellular Biophysical Features with a Standard Optical Microscope
14:09

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16.0K
Easy and Accurate Mechano-profiling on Micropost Arrays
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Area of Science:

  • Cell Biology
  • Biophysics
  • Surface Science

Background:

  • Cell surface protein and glycan dimensions influence cell-cell interactions and receptor accessibility.
  • Accurate, high-resolution measurement of these molecular heights on native cell membranes is challenging.

Purpose of the Study:

  • To develop and validate a novel, rapid method for high-resolution measurement of cell surface molecular heights.
  • To investigate the relationship between molecular dimensions and cell function under varying conditions.

Main Methods:

  • Developed cell surface optical profilometry (CSOP) using fluorophore localization at molecule tips and bases.
  • Employed radial averaging across numerous molecules for nanometer height resolution.
  • Combined experimental measurements with molecular dynamics simulations.

Main Results:

  • Quantified heights of specific proteins and average protein/glycan heights on native cell membranes.
  • Demonstrated that protein height is less than contour length due to thermal motion.
  • Showed that molecular height is influenced by local surface and solution crowding.

Conclusions:

  • CSOP provides a versatile tool for profiling cell surface molecular architecture.
  • Understanding molecular dimensions is crucial for deciphering cell function and interactions.
  • The method can be adapted to profile various cell surface components using antibodies and lectins.