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Genome sequence analysis of a novel Enterobacter cloacae phage, Ec_L1, belonging to the genus Eclunavirus

Hongyu Ren1, Zhen Li2,3, Xiaoyu Li1

  • 1School of Bioengineering, Dalian University of Technology, Dalian, 116024, People's Republic of China.

Archives of Virology
|June 10, 2020
PubMed

Insights

Researchers isolated a novel bacteriophage, Ec_L1, from hospital sewage. This phage targets antibiotic-resistant bacteria and has been classified into a new genus, Eclunavirus, offering potential for phage therapy.

Area of Science:

  • Microbiology
  • Virology
  • Genomics

Background:

  • Bacteriophages (phages) are viruses that infect bacteria and show promise for treating infections caused by antibiotic-resistant bacteria.
  • The rise of multidrug-resistant pathogens necessitates the exploration of alternative therapeutic strategies like phage therapy.

Purpose of the Study:

  • To isolate and characterize a novel bacteriophage targeting Enterobacter cloacae.
  • To determine the genomic features and taxonomic classification of the isolated phage Ec_L1.
  • To evaluate the potential of phage Ec_L1 in combating antibiotic-resistant bacterial infections.

Main Methods:

  • Isolation of bacteriophage Ec_L1 from hospital sewage sludge samples.
  • Whole-genome sequencing and bioinformatic analysis of phage Ec_L1.
  • Comparative genomic analysis with known bacteriophages.
  • Taxonomic classification according to the International Committee on Taxonomy of Viruses (ICTV).

Main Results:

  • Isolation of a viable Enterobacter cloacae phage, designated Ec_L1.
  • The Ec_L1 genome is 51,894 bp with 48.24% G+C content, encoding 85 putative proteins.
  • Phage Ec_L1 shares genomic characteristics with T1-like phages and belongs to the subfamily Tunavirinae.
  • Ec_L1 is designated the type member of the new genus 'Eclunavirus' by the ICTV.

Conclusions:

  • Phage Ec_L1 represents a novel addition to the bacteriophage repertoire for potential therapeutic applications.
  • The genomic characterization and classification of Ec_L1 provide a foundation for further studies in phage therapy.
  • The establishment of the 'Eclunavirus' genus highlights the diversity of phages and their evolutionary significance.

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