Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Complement activation and serum beta-glucuronidase level during hemodialysis.

N Kural1, U Saatçi, A Bakkaloglu

  • 1Faculty of Medicine, Anadolu University, Eskişehir, Turkey.

Child Nephrology and Urology
|January 1, 1988
PubMed
Summary

Hemodialysis patients experienced a drop in neutrophils and complement C3 levels at the start of dialysis. This complement activation and resulting leukopenia correlated with increased beta-glucuronidase activity, raising infection risk.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

The Effect of Luer-Lock Adapter on Hemolysis Rates in the Emergency Department.

Nigerian journal of clinical practice·2025
Same author

High sensitive C-reactive protein: a new marker for urinary tract infection, VUR and renal scar.

European review for medical and pharmacological sciences·2013
Same author

Follow-up of patients with juvenile nephronophthisis after renal transplantation: a single center experience.

Transplantation proceedings·2011
Same author

Henoch-Schönlein nephritis: a nationwide study.

Nephron. Clinical practice·2009
Same author

The analysis of interleukin-1 receptor antagonist and interleukin-1beta gene polymorphisms in Turkish FMF patients: do they predispose to secondary amyloidosis?

Clinical and experimental rheumatology·2008
Same author

MEFV mutations in systemic onset juvenile idiopathic arthritis.

Rheumatology (Oxford, England)·2008

Area of Science:

  • Nephrology
  • Immunology
  • Biochemistry

Background:

  • Chronic hemodialysis is a life-sustaining treatment for kidney failure.
  • Hemodialysis can trigger inflammatory responses and immune system alterations.
  • Understanding these changes is crucial for managing patient health and preventing complications.

Purpose of the Study:

  • To investigate the impact of hemodialysis initiation on peripheral white blood cell counts, complement system activation, and serum beta-glucuronidase activity in pediatric patients.
  • To explore the relationship between complement activation, leukopenia, and beta-glucuronidase levels during hemodialysis.
  • To assess the clinical implications of these biochemical changes, particularly regarding infection risk.

Main Methods:

  • Peripheral white blood cell counts were measured in 15 pediatric hemodialysis patients (8-10 years old).

Related Experiment Videos

  • Complement C3 and C4 levels were analyzed.
  • Serum beta-glucuronidase activity was determined at dialysis initiation, 15 minutes post-initiation, and at the end of dialysis.
  • Changes were compared to predialysis values.

    • Main Results:

    • A significant decline in neutrophils was observed immediately after dialysis initiation.
    • Complement C3 levels decreased concurrently, while C4 levels remained unchanged.
    • Plasma beta-glucuronidase activity increased, correlating with complement activation and leukopenia.
    • Granulocytopenia was identified as a significant risk factor for hemodialysis-associated infections.

    Conclusions:

    • Hemodialysis initiation triggers complement activation and acute leukopenia in pediatric patients.
    • Elevated serum beta-glucuronidase activity is a marker of complement activation and leukopenia during hemodialysis.
    • These findings highlight the increased susceptibility to infection in hemodialysis patients due to granulocytopenia.