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Nontoxic concentration of antibiotics for intravitreal use--evaluated by human in-vitro ERG
1Department of Ophthalmology, Kanazawa University School of Medicine, Japan.
Abstract:
The effects of penicillin G (PC-G) sodium, procaine PC-G, cloxacillin sodium (MCIPC), disodium sulbenicillin (SBPC), cefazolin sodium (CEZ), gentamicin sulfate (GM) and fosfomycin sodium (FOM) on the electroretinogram (ERG) of the human in-vitro eye-cup were studied. The oscillatory potentials (OPs) were selectively and greatly suppressed by 1.0 mM PC-G sodium. The OPs and c-wave were suppressed by 0.85 mM procaine PC-G. The b-wave and OPs were slightly suppressed by 1.0 mM MCIPC. The a-wave, b-wave, OPs and c-wave were not deteriorated by 1.0 mM SBPC. The OPs appeared to be selectively suppressed by 1.0 mM CEZ. The b-wave was suppressed and the peak latencies of the OPs were delayed by 184 micrograms/ml (approximately 0.4 mM) GM. The amplitudes of the a-wave and c-wave were slightly enhanced and their peak latencies were slightly delayed by 184 micrograms/ml GM. The a-wave, b-wave, OPs and c-wave were not deteriorated by 1.0 mM FOM. The results of the present study on the human retina were comparable to those on the albino rabbit retina in our previous studies.
Insights
Penicillin G and related antibiotics affect human retinal function. Fosfomycin sodium and disodium sulbenicillin showed no adverse effects on the electroretinogram (ERG), suggesting potential safety for ocular use.
Area of Science:
- Ophthalmology
- Pharmacology
- Neuroscience
Background:
- Antibiotics are crucial in treating ocular infections.
- Understanding their impact on retinal function is vital for patient safety.
- Previous studies explored rabbit retina responses to these agents.
Purpose of the Study:
- To investigate the in-vitro effects of various antibiotics on the human electroretinogram (ERG).
- To assess the safety and potential retinal toxicity of specific antibiotics.
- To compare human retinal responses with previous findings in albino rabbits.
Main Methods:
- Human eye-cup preparations were utilized for in-vitro testing.
- Electroretinograms (ERGs) were recorded to measure retinal function.
- The effects of penicillin G sodium, procaine PC-G, cloxacillin sodium, disodium sulbenicillin, cefazolin sodium, gentamicin sulfate, and fosfomycin sodium were evaluated at specific concentrations.
Main Results:
- Penicillin G sodium and procaine PC-G significantly suppressed oscillatory potentials (OPs) and c-wave.
- Cloxacillin sodium and cefazolin sodium showed selective suppression of OPs.
- Gentamicin sulfate affected the b-wave and OPs, with minor effects on a-wave and c-wave amplitudes and latencies.
- Disodium sulbenicillin and fosfomycin sodium did not deteriorate a-wave, b-wave, OPs, or c-wave.
- Human retinal responses mirrored those observed in albino rabbits.
Conclusions:
- Certain antibiotics, including penicillin G and its derivatives, can negatively impact human retinal function.
- Disodium sulbenicillin and fosfomycin sodium appear safe for human retinal function in vitro.
- Findings support the comparable electroretinographic effects between human and rabbit retinas for these antibiotics.