Nonomuraea terrae sp. nov., isolated from arid soil

During the course of isolating rare actinobacteria from unexplored habitats, strain CH32^T was obtained from an arid soil sample in eastern Anatolia, Turkey. Polyphasic characterization and comprehensive genome analyses showed that the strain is a member of the genus Nonomuraea and it is closely related to Nonomuraea gerenzanensis ATCC 39727^T, Nonomuraea polychroma DSM 43925^T and Nonomuraea maritima FXJ7.203^T with gene identity level of 98.7%, 98.2% and 98.1%, respectively. The whole-cell hydrolysates contain meso-diaminopimelic acid as diagnostic diaminoacid and glucose, ribose, galactose, mannose and madurose as whole cell sugars. The predominant menaquinones are MK-9(H₄), MK-9(H₆) and MK-9(H₂) while MK-9 exists as minor component. The polar lipid profile consists of diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol, phosphatidylinositol, glycolipid, glycophospholipids, phospholipids and unidentified lipids. The major cellular fatty acids are iso-C_16:0 and C_17:0 10-methyl. The total genome size is about 9.6 Mb and the G + C content is 71.0%. The genome contains biosynthetic gene clusters encoding for terpenes, siderophores, a type III polyketide synthase, a non-ribosomal polypeptide synthetase and a bacteriocin. The genome-based comparisons of the strain with its phylogenetic neighbours, as indicated by digital DNA-DNA hybridization and average nucleotide identity analyses, reveal that strain CH32^T (= JCM 33876^T = KCTC 49368^T) is a novel member of the genus Nonomuraea, for which Nonomuraea terrae sp. nov. is proposed.