Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Atomic Force Microscopy01:08

Atomic Force Microscopy

4.2K
Atomic force microscopy (AFM) is a type of scanning probe microscopy that can analyze topographic details of various specimens like ceramics, glass, polymers, and biological samples. AFM offers over 1000 times more resolution than the optical imaging system. Images generated from AFM are three-dimensional surface profiles, offering an advantage over the flat, two-dimensional images from other imaging techniques.
The AFM Probe
The probe is regarded as the heart of any AFM setup and comprises the...
4.2K
Studying the Cytoskeleton01:17

Studying the Cytoskeleton

8.4K
The cytoskeletal architecture can be studied using different microscopic and biochemical techniques. Electron microscopy was instrumental in discovering the cytoskeletal architecture around the 1960s, which allowed obtaining structural information at a high-resolution level. However, the sample preparation procedure often limits this ability in biological samples. Several protocols have been developed over the years to optimize sample preparation. In one of the protocols known as rotary...
8.4K
Overview of Microscopy Techniques01:22

Overview of Microscopy Techniques

14.5K
The early pioneers of microscopy opened a window into the invisible world of microorganisms. In 1830, Joseph Jackson Lister created an essentially modern light microscope. The 20th century saw the development of microscopes that leveraged nonvisible light, such as fluorescence microscopy that uses an ultraviolet light source and electron microscopy that uses short-wavelength electron beams. These advances significantly improved magnification, image resolution, and contrast. By comparison, the...
14.5K
Three-Dimensional Microscopy in Microbiology01:28

Three-Dimensional Microscopy in Microbiology

678
Three-dimensional imaging techniques are essential in cell biology, allowing researchers to visualize intricate cellular structures with high resolution. Two prominent methods, Differential Interference Contrast Microscopy (DIC) and Confocal Scanning Laser Microscopy (CSLM), provide distinct advantages for imaging live and thick specimens, respectively.Differential Interference Contrast MicroscopyDIC microscopy enhances contrast in transparent, unstained samples by converting phase...
678

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Investigating the Effect of Ginger-Derived Nanovesicles on the Growth and Metabolic Activity of <i>Bacteroides thetaiotaomicron</i>: An Isothermal Microcalorimetric Study.

Journal of extracellular biology·2026
Same author

When artificial intelligence meets protein research.

Open research Europe·2025
Same author

Platelets as key cells in endometriosis patients: Insights from small extracellular vesicles in peritoneal fluid and endometriotic lesions analysis.

FASEB journal : official publication of the Federation of American Societies for Experimental Biology·2024
Same author

Structures of Oligomeric States of Tau Protein, Amyloid-β, α-Synuclein and Prion Protein Implicated in Alzheimer's Disease, Parkinson's Disease and Prionopathies.

International journal of molecular sciences·2024
Same author

A Novel Tetrahydroacridine Derivative with Potent Acetylcholinesterase Inhibitory Properties and Dissociative Capability against Aβ42 Fibrils Confirmed by In Vitro Studies.

International journal of molecular sciences·2024
Same author

<i>N</i>-Acetylcysteine-Loaded Magnetic Nanoparticles for Magnetic Resonance Imaging.

International journal of molecular sciences·2023

Related Experiment Video

Updated: Dec 18, 2025

Visualization of Recombinant DNA and Protein Complexes Using Atomic Force Microscopy
08:30

Visualization of Recombinant DNA and Protein Complexes Using Atomic Force Microscopy

Published on: July 18, 2011

22.9K

Atomic force microscopy as an imaging tool to study the bio/nonbio complexes.

Z Bednarikova1, Z Gazova1, F Valle2

  • 1Slovak Academy of Science, Institute of Experimental Physics, Kosice, Slovakia.

Journal of Microscopy
|June 11, 2020
PubMed
Summary

Atomic force microscopy (AFM) reveals how biomacromolecules interact with nanomaterials, offering crucial insights for nanomedicine and safety assessments. This review highlights AFM

Keywords:
AFMAmyloid fibrilsDNAbiomoleculesspectroscopy

More Related Videos

Atomic Force Microscopy of Red-Light Photoreceptors Using PeakForce Quantitative Nanomechanical Property Mapping
14:13

Atomic Force Microscopy of Red-Light Photoreceptors Using PeakForce Quantitative Nanomechanical Property Mapping

Published on: October 24, 2014

12.0K
Atomic Force Microscopy Combined with Infrared Spectroscopy as a Tool to Probe Single Bacterium Chemistry
08:51

Atomic Force Microscopy Combined with Infrared Spectroscopy as a Tool to Probe Single Bacterium Chemistry

Published on: September 15, 2020

4.5K

Related Experiment Videos

Last Updated: Dec 18, 2025

Visualization of Recombinant DNA and Protein Complexes Using Atomic Force Microscopy
08:30

Visualization of Recombinant DNA and Protein Complexes Using Atomic Force Microscopy

Published on: July 18, 2011

22.9K
Atomic Force Microscopy of Red-Light Photoreceptors Using PeakForce Quantitative Nanomechanical Property Mapping
14:13

Atomic Force Microscopy of Red-Light Photoreceptors Using PeakForce Quantitative Nanomechanical Property Mapping

Published on: October 24, 2014

12.0K
Atomic Force Microscopy Combined with Infrared Spectroscopy as a Tool to Probe Single Bacterium Chemistry
08:51

Atomic Force Microscopy Combined with Infrared Spectroscopy as a Tool to Probe Single Bacterium Chemistry

Published on: September 15, 2020

4.5K

Area of Science:

  • Bionanotechnology
  • Materials Science
  • Biophysics

Background:

  • Atomic force microscopy (AFM) is a key technique for studying bio/nonbio complexes, complementing X-ray crystallography and electron microscopy.
  • Understanding biomacromolecule-nanomaterial interactions is vital for nanomedicine applications and nanomaterial safety evaluations.
  • Bio/nonbio complexes exhibit diverse stability and composition, necessitating advanced imaging and analysis methods.

Purpose of the Study:

  • To review the significance of AFM in studying biological aspects of selected bio/nonbio assemblies.
  • To highlight the insights gained from AFM operating in air for structure and function analysis.
  • To showcase AFM's role in bionanotechnology and its applications in nanomedicine.

Main Methods:

  • Exploitation of Atomic Force Microscopy (AFM) operating in ambient air conditions.
  • Imaging of three-dimensional topography at nanoscale resolution (10⁻⁹ m).
  • Analysis of structural, functional, and mechanical properties of biological and material samples.

Main Results:

  • AFM provides detailed structural and functional data on bio/nonbio assemblies, including proteins, lipids, DNA, and cells.
  • AFM offers unique and unexpected insights into the behavior of these complexes.
  • AFM demonstrates utility as a nanodiagnostic tool for patient cells in nanomedicine.

Conclusions:

  • AFM is a powerful microscopy technology for nanoscale biological and material studies with minimal sample preparation.
  • AFM elucidates biomacromolecule-nanomaterial interactions under various environmental conditions.
  • AFM applications are becoming well-established in nanomedicine for diagnostics and safety assessments.