Related Experiment Video

Updated: Dec 18, 2025

Method for the Isolation and Identification of mRNAs, microRNAs and Protein Components of Ribonucleoprotein Complexes from Cell Extracts using RIP-Chip
13:34

Method for the Isolation and Identification of mRNAs, microRNAs and Protein Components of Ribonucleoprotein Complexes from Cell Extracts using RIP-Chip

Published on: September 29, 2012

28.0K

Prognostic Significance of CHIP and RIPK3 in Non-Small Cell Lung Cancer

Jisup Kim1, Joon-Yong Chung2, Young Soo Park1

  • 1Department of Pathology, Asan Medical Center, University of Ulsan College of Medicine, Seoul 05505, Korea.

Cancers
|June 12, 2020
PubMed

Insights

In non-small cell lung cancer (NSCLC), higher expression of CHIP correlates with longer survival, while higher RIPK3 expression is linked to shorter survival. These findings suggest CHIP and RIPK3 have opposing prognostic values in NSCLC patients.

Area of Science:

  • Oncology
  • Molecular Biology
  • Cancer Research

Background:

  • Necroptosis, regulated by RIPK3, has a complex role in tumor progression.
  • CHIP, an E3 ubiquitin ligase, modulates necroptosis by targeting RIPK3 for degradation.

Purpose of the Study:

  • To investigate the prognostic significance of Receptor-Interacting Protein Kinase 3 (RIPK3) and Carboxyl Terminus of Hsp70-Interacting Protein (CHIP) expression in non-small cell lung cancer (NSCLC).

Main Methods:

  • Analysis of RIPK3 and CHIP expression levels in 404 NSCLC patients.
  • Correlation of expression levels with overall survival (OS) and disease-free survival (DFS).
  • Multivariate analysis to determine independent prognostic factors.

Main Results:

  • CHIP expression was positively correlated with longer OS, while RIPK3 expression was associated with shorter OS.
  • RIPK3 positivity suggested potential radioresistance in NSCLC patients receiving adjuvant radiotherapy.
  • CHIP expression emerged as an independent favorable prognostic factor in multivariate analysis.

Conclusions:

  • CHIP expression is a favorable prognostic indicator in NSCLC.
  • RIPK3 expression is an adverse prognostic indicator in NSCLC.
  • The opposing roles of CHIP and RIPK3 highlight their significance in NSCLC prognosis and potential therapeutic targeting.
Keywords:
carboxyl terminus of Hsp70-interacting protein (CHIP)necroptosisnon-small cell lung cancerprognosisreceptor interacting serine/threonine kinase 3 (RIPK3)

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