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MicroRNA-155 complementation on a chemically functionalized dual electrode surface for determining breast cancer
Subash C B Gopinath1,2, Veeradasan Perumal3, Shijin Xuan4
1School of Bioprocess Engineering, Universiti Malaysia Perlis, 02600 Arau, Perlis Malaysia.
3 Biotech
|June 12, 2020
Summary
This study quantifies microRNA-155 expression for breast cancer progression. The developed biosensor accurately detects microRNA-155, aiding in early breast cancer diagnosis.
Area of Science:
- Biomedical Engineering
- Molecular Biology
- Oncology
Background:
- MicroRNA-155 (miRNA-155) is implicated in various cancers, including breast cancer.
- Accurate quantification of miRNA expression is crucial for understanding cancer progression and developing diagnostic tools.
Purpose of the Study:
- To correlate and quantify microRNA-155 expression in relation to breast cancer progression.
- To develop a sensitive and selective method for detecting miRNA-155.
Main Methods:
- Utilized an interdigitated dual electrode surface with immobilized capture probes for miRNA-155 identification.
- Employed sequence complementation for specific miRNA binding and detection.
- Assessed assay sensitivity, limit of detection, and sequence selectivity against various mismatches.
Main Results:
- Achieved a high sensitivity of 1 femtomolar (fM) for miRNA-155 detection.
- Established a limit of detection between 1 and 10 fM.
- Demonstrated high sequence selectivity, with no significant binding observed for sequences with single mismatches, triple mismatches, or noncomplementary bases.
Conclusions:
- The developed method enables selective and sensitive determination of the microRNA-155 sequence.
- This biosensor technology holds potential for the early diagnosis and monitoring of breast cancer progression.
