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Microinjection of Superior Cervical Ganglion Neurons for Studying Axon Degeneration
Jonathan Gilley1, Andrea Loreto2
1Department of Clinical Neurosciences, John van Geest Centre for Brain Repair, University of Cambridge, Cambridge, UK. jg792@cam.ac.uk.
Abstract:
Primary cultures of neurons of the peripheral nervous system have been successfully used for studying many aspects of neuronal development and survival, including investigations into the mechanisms of axon degeneration. In this chapter, we describe how to prepare and microinject dissociated cultures of sympathetic neurons of the superior cervical ganglion (SCG) specifically for use in highly controlled and targeted assays of axon survival and degeneration.
