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Updated: Dec 18, 2025
In Vivo Methods to Assess Retinal Ganglion Cell and Optic Nerve Function and Structure in Large Animals
Published on: February 26, 2022
Ex Vivo Studies of Optic Nerve Axon Electrophysiology
Chinthasagar Bastian1, Sylvain Brunet1, Selva Baltan2,3
1Department of Neurosciences, Lerner Research Institute, Cleveland Clinic Foundation, Cleveland, OH, USA.
Abstract:
The use of ex vivo compound action potential (CAP) recordings from intact optic nerves is an ideal model to study white matter function without the influence of gray matter. Here, we describe how freshly dissected optic nerves are placed in a humidified recording chamber and how evoked CAPs are recorded and monitored in real time for up to 10 h. Evoked CAP recordings allow for white matter to be studied under acute challenges such as anoxia, hypoxia, aglycemia, and ischemia.
