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Patterning of Microorganisms and Microparticles through Sequential Capillarity-assisted Assembly
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Bactericidal nanopatterns generated by block copolymer self-assembly.

R Fontelo1, D Soares da Costa1, R L Reis2

  • 13B's Research Group, I3Bs - Research Institute on Biomaterials, Biodegradables and Biomimetics, University of Minho, Headquarters of the European Institute of Excellence on Tissue Engineering and Regenerative Medicine, AvePark, Parque de Ciência e Tecnologia, Zona Industrial da Gandra, 4805-017 Barco, Guimarães, Portugal; ICVS/3B's-PT Government Associate Laboratory, Braga Guimarães, Portugal.

Acta Biomaterialia
|June 12, 2020
PubMed
Summary
This summary is machine-generated.

New nanopatterned surfaces kill bacteria by mechanical stress on cell walls. These surfaces are non-cytotoxic, offering a promising alternative to antibiotics for implantable materials and devices.

Keywords:
Antibacterial polymer surfacesBlock copolymersCell adhesionNanopatternsPS-b-P2VP

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Area of Science:

  • Materials Science
  • Biotechnology
  • Surface Chemistry

Background:

  • Antibiotic resistance necessitates novel strategies against bacterial infections, especially in biomaterial implantation.
  • Existing bactericidal surfaces often harm mammalian cells or are limited in material applicability and scalability.
  • Nanopatterned surfaces offer a promising avenue, but scalable and versatile fabrication methods are needed.

Purpose of the Study:

  • To develop and characterize bactericidal nanopatterned surfaces using block copolymer self-assembly.
  • To investigate the relationship between nanotopography, surface chemistry, and bactericidal efficacy against Escherichia coli.
  • To assess the cytocompatibility of these nanopatterned surfaces for potential biomedical applications.

Main Methods:

  • Fabrication of nanotopographies via self-assembly of polystyrene-block-poly(2-vinylpyridine) (PS-b-P2VP) using spin-coating and solvent vapor annealing.
  • Characterization of surface morphology and chemical composition.
  • Evaluation of bactericidal activity against Escherichia coli and assessment of mammalian cell cytotoxicity.

Main Results:

  • Distinct nanotopographies, including cylindrical and micellar patterns, were successfully generated.
  • Cylindrical nanotopographies with exposed PS and P2VP blocks exhibited enhanced bactericidal effects on Escherichia coli compared to micellar patterns.
  • The primary mechanism of bacterial death was identified as mechanical stress on the bacterial cell wall.
  • The developed nanopatterned surfaces demonstrated no cytotoxicity towards mammalian cells.

Conclusions:

  • Block copolymer self-assembly provides a versatile and scalable method for creating bactericidal surfaces.
  • Nanopatterned surfaces can effectively kill bacteria via mechanical disruption of cell walls without harming mammalian cells.
  • These non-cytotoxic, bactericidal surfaces are suitable for coating implantable materials and devices, addressing the challenge of antibiotic resistance.