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Updated: Dec 18, 2025
Author Spotlight: Exploring Cellular Zinc Regulation Through ZnT1 Functionality
Published on: June 2, 2023
A novel peptide-based fluorescent probe for sensitive detection of zinc (II) and its applicability in live cell
Peng Wang1, Sihan Wang1, Li Chen1
1Chemical Synthesis and Pollution Control Key Laboratory of Sichuan Province, College of Chemistry and Chemical Engineering, China West Normal University, Shida Road 1#, Nanchong 637009, PR China.
Abstract:
In this work, we report SPSS synthesis of a new peptide-based fluorescent probe (L) capable of detecting Zn2+ with little interference in 100% aqueous solutions at physiological pH. Furthermore, L showed excellent sensitivity, with a detection limit of 26.77 nM. The 2:1 binding ratio between L and Zn2+ was determined using fluorometric titration, Job's plot and ESI-MS analyses. The "off-on-off" type fluorescence change of L was demonstrated by alternately adding Zn2+ and EDTA based on a formation-separation process of the complex, indicating that L could serve as a reversible probe. Moreover, MTT studies demonstrated that L has low biotoxicity, and could be successfully used for detection of Zn2+ and EDTA in live cells.
