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Related Experiment Video

Updated: Dec 18, 2025

A Simple Flow Cytometric Method to Measure Glucose Uptake and Glucose Transporter Expression for Monocyte Subpopulations in Whole Blood

A Simple Flow Cytometric Method to Measure Glucose Uptake and Glucose Transporter Expression for Monocyte Subpopulations in Whole Blood

Published on: August 12, 2016

Glucose transport in lymphocytes.

Florian Lang^1,2, Yogesh Singh³, Madhuri S Salker⁴

¹Department of Physiology, Eberhard Karl University, Tubingen, Germany. florian.lang@uni-tuebingen.de.

Pflugers Archiv : European Journal of Physiology

|June 13, 2020

Summary

Sodium-glucose cotransporter 1 (SGLT1) is crucial for lymphocyte glucose uptake and function, especially in low-glucose conditions. Its absence impairs host defense against bacterial infections, highlighting its importance in immunity.

Keywords:

Bacterial infection Cancer Cytotoxic T lymphocytes Energy depletion GLUT Janus kinases Jurkat T cells SGLT1

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Published on: November 7, 2010

Area of Science:

Immunology
Cell Biology
Metabolism

Background:

Lymphocyte glucose uptake occurs via GLUT transporters and the Na+-coupled SGLT1.
SGLT1 accumulates glucose against gradients, vital in low-glucose environments.
Lymphocytes rely on glycogen-dependent glycolysis in hypoxic conditions like tumors and infections.

Purpose of the Study:

To investigate the role of SGLT1 in lymphocyte glucose uptake and function.
To determine the impact of SGLT1 deficiency on host defense mechanisms.
To clarify the significance of glucose transport for lymphocyte survival and immune response.

Main Methods:

Analysis of glucose transport mechanisms in lymphocytes.
Investigation of signaling pathways regulating SGLT1.
Assessment of host defense in SGLT1 knockout mouse models.

Main Results:

SGLT1 facilitates glucose uptake, particularly at low extracellular glucose concentrations.
Signaling pathways including PKA, PKC, SGK1, AMPK, JAK2, and JAK3 regulate SGLT1.
Genetic knockout of SGLT1 in mice impaired bacterial clearance and led to lethal infections.

Conclusions:

SGLT1 plays a critical role in lymphocyte glucose metabolism and function, especially under hypoxic stress.
The absence of SGLT1 may compromise lymphocyte survival and immune response.
Further research is needed to fully elucidate the role of GLUTs and SGLT1 in lymphocyte-mediated host defense.