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Purification of the Dynamin-Related Protein Vps1 Using Mammalian and Bacterial Expression Systems
Natalia V Varlakhanova1, Marijn G J Ford2
1Department of Cell Biology, University of Pittsburgh School of Medicine, Pittsburgh, PA, USA.
Methods in Molecular Biology (Clifton, N.J.)
|June 13, 2020
Abstract:
The dynamin-related proteins (DRPs) are self-assembling membrane remodeling machines that are indispensable for fundamental cellular trafficking and homeostatic processes. We describe in this chapter protocols developed in our laboratory for purification of full-length and minimal constructs of Chaetomium thermophilum Vps1, the model fungal DRP, using mammalian and Escherichia coli expression systems.

