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Related Experiment Video

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Pollen Grain Counting Using a Cell Counter.

Hiroyuki Kakui1,2, Misako Yamazaki1, Naoto-Benjamin Hamaya1

  • 1Department of Evolutionary Biology and Environmental Studies, University of Zurich, Zurich, Switzerland.

Methods in Molecular Biology (Clifton, N.J.)
|June 13, 2020
PubMed
Summary
This summary is machine-generated.

Counting plant pollen is faster with a cell counter. This method significantly reduces time for pollen analysis and viability assessment in crop breeding.

Keywords:
Arabidopsis kamchaticaArabidopsis thalianaCell counter (CASY cell counter)Pollen numberPollen sizePollen viabilityWheat (Triticum aestivum)

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Area of Science:

  • Plant reproductive biology
  • Agricultural science
  • Biotechnology

Background:

  • Pollen grain count is crucial for plant reproduction and crop breeding.
  • Traditional methods for pollen counting are time-consuming and labor-intensive.

Purpose of the Study:

  • To introduce a novel, rapid method for counting pollen grains using a cell counter.
  • To assess pollen viability and size distribution efficiently.

Main Methods:

  • Utilized a cell counter for pollen grain enumeration.
  • Developed a protocol applicable to various plant species, including Arabidopsis and wheat.
  • Measured pollen particle size and quantified normal versus aborted pollen.

Main Results:

  • The cell counter method reduced counting time to 3 minutes per flower, over five times faster than traditional methods.
  • The technique accurately quantifies pollen across a wide range (0–20,000 particles).
  • Enabled separate quantification of normal and aborted pollen based on size and membrane characteristics.

Conclusions:

  • Cell counter technology offers a significantly faster and more efficient approach to pollen analysis.
  • This method enhances pollen viability assessment for crop breeding programs.
  • The technique is versatile and applicable to diverse plant species.