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Updated: Dec 18, 2025

Imaging Spatial Reorganization of a MAPK Signaling Pathway Using the Tobacco Transient Expression System
Published on: March 20, 2016
Analyzing the Impact of Protein Overexpression on Ca2+ Dynamics and Development in Tobacco Pollen Tubes
Chunxia Zhang1,2, Leonie Steinhorst1, Jörg Kudla3
1Institute of Plant Biology and Biotechnology, University of Münster, Münster, Germany.
Abstract:
Overexpression of RFP-tagged proteins in growing tobacco pollen tubes together with the genetically encoded Ca2+ sensor YC3.6 allows to analyze localization and dynamics of the protein of interest, as well as the impact of its overexpression on Ca2+ dynamics and pollen tube growth. Here, we describe a step-by-step instruction for transient transformation of N. tabacum pollen and subsequent in vitro germination and Ca2+ imaging.
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