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Real Time RT-PCR

Real Time RT-PCR

Real-time reverse transcription-polymerase chain reaction, or Real-time RT-PCR, is an analytical tool used to determine the expression level of target genes. The method involves converting mRNA to complementary DNA with the help of an enzyme known as reverse transcriptase, followed by the PCR amplification of the cDNA. These two processes can be performed simultaneously in a single tube or separately as a two-step reaction.
The real-time quantification of the number of amplified products is...

PCR

PCR

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Related Experiment Video

Updated: Dec 18, 2025

Two-Step Reverse Transcription Droplet Digital PCR Protocols for SARS-CoV-2 Detection and Quantification

Two-Step Reverse Transcription Droplet Digital PCR Protocols for SARS-CoV-2 Detection and Quantification

Published on: March 31, 2021

COVID reverse transcriptase PCR in private laboratories: From theory to reality

M Autissier¹, G Guiraud², Y Lévy¹

¹Université Claude Bernard Lyon 1, faculté de médecine Lyon est, collège universitaire de médecine générale, 8, avenue Rockefeller, 69008 Lyon, France.

Medecine Et Maladies Infectieuses

|June 13, 2020

Summary

No abstract available in PubMed .

Keywords:

COVID-19 Reverse transcriptase PCR

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