Genetics of colour variation in wild versus cultured queen loach, Botia dario (Hamilton, 1822)
Related Experiment Video
Updated: Dec 18, 2025

Dyeing Insects for Behavioral Assays: the Mating Behavior of Anesthetized Drosophila
Published on: April 22, 2015
Sagar Chandra Mandal1, Partha Sarathi Tripathy1, Ananya Khatei1
1College of Fisheries, Central Agricultural University (Imphal), Lembucherra, Tripura 799210, India.
Abstract:
The Queen loach (Botia dario), an ornamental fish species having export potential, belongs to family Cobitidae of order Cypriniformes. The dull colouration in captive condition as compared to nature is a drawback in ornamental fisheries. We report the first comparative transcriptomic analysis of Cultured (CBD) and Natural (NBD) B. dario using bioinformatics tools. Total 26 and 7 key genes for melanin and carotenoid colouration were found, respectively. KEGG pathway annotations of the genes were carried out, to annotate and describe their relevance for pigmentation. The qPCR validation of genes confirmed their expression pattern in the skin and muscle. Differential expression of, slc7a11, asip1, mc1r, dct, tyrp1a, tyr, bcdo2, csf1r, plin2, gsta2, star3 and stard5 in the skin and muscle tissues revealed the reasons for wild versus cultured colour variation. The molecular data was further supported by low yellowness and redness values of CBD skin and muscle in a colorimeter.
More Related Videos
Related Concept Videos
Background and Environment Affect Phenotype
An example of how genetic background affects phenotype can be seen in horses. The Extension gene in horses is responsible for their coat color. A wild-type gene (EE) produces black pigment in the coat, while a mutant gene (ee) produces red pigment. A...
Testing a Claim about Population Proportion
There are two methods of testing a claim about a population proportion: (1) Using the sample proportion from the data where a binomial distribution is approximated to the normal distribution and (2) Using the binomial probabilities calculated from the data.
The first method uses normal distribution as an approximation to the binomial distribution. The requirements are as follows: sample size is large...
Genetics of Speciation
Incomplete Dominance
Genetic Lingo
Genetic Variation
Genes exist in different versions called alleles,...

