Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

RNA-seq03:21

RNA-seq

11.6K
RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases. 
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while...
11.6K
Next-generation Sequencing03:00

Next-generation Sequencing

97.2K
The first human genome sequencing project cost $2.7 billion and was declared complete in 2003, after 15 years of international cooperation and collaboration between several research teams and funding agencies. Today, with the advent of next-generation sequencing technologies, the cost and time of sequencing a human genome have dropped over 100 fold.
Next-Generation Sequencing Methods
Although all next-generation methods use different technologies, they all share a set of standard features....
97.2K
Sanger Sequencing01:57

Sanger Sequencing

771.6K
DNA sequencing is a fundamental technique that is routinely used in the biological sciences. This method can be applied to a range of questions at different scales - from the sequencing of a cloned DNA fragment or the study of a mutation in a gene up to whole-genome sequencing. However, despite the widespread use of sequencing today, it was not until 1977 that Fredrick Sanger and his collaborators developed the chain-termination method to decode DNA sequences. It relies on the separation of a...
771.6K
Maxam-Gilbert Sequencing01:05

Maxam-Gilbert Sequencing

12.4K
In the same year as the discovery of the Sanger sequencing method, another group of scientists, Allan Maxam and Walter Gilbert, demonstrated their chemical-cleavage method for DNA sequencing. The Maxam-Gilbert method relies on using different chemicals that can cleave the DNA sequence at specific sites, the separation of resulting DNA fragments of variable size using electrophoresis, and deciphering the DNA sequence from the resulting gel bands.
Challenges of the Maxam-Gilbert Method
The...
12.4K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Molecular detection of insecticide resistance markers in Aedes aegypti (Diptera: Culicidae) populations from Haiti.

Journal of medical entomology·2026
Same author

Stereotactic magnetic resonance imaging-guided adaptive radiotherapy: a pooled analysis of a master prospective trial.

Journal of the National Cancer Institute·2025
Same author

Evaluation of successive DNA extractions from cotton and flocked swabs.

Forensic science, medicine, and pathology·2025
Same author

<i>RPS19</i> and <i>RPL5</i>, the most commonly mutated genes in Diamond Blackfan anemia, impact DNA double-strand break repair.

bioRxiv : the preprint server for biology·2024
Same author

Integrated epigenomic exposure signature discovery.

Epigenomics·2024
Same author

Lung sparing in MR-guided non-adaptive SBRT treatment of peripheral lung tumors.

Biomedical physics & engineering express·2024

Related Experiment Video

Updated: Dec 18, 2025

High-throughput Identification of Gene Regulatory Sequences Using Next-generation Sequencing of Circular Chromosome Conformation Capture 4C-seq
09:06

High-throughput Identification of Gene Regulatory Sequences Using Next-generation Sequencing of Circular Chromosome Conformation Capture 4C-seq

Published on: October 5, 2018

10.7K

Sequence-based autosomal STR characterization in four US populations using PowerSeq™ Auto/Y system.

Deborah S B S Silva1, Melissa K Scheible2, Sarah F Bailey3

  • 1Battelle Memorial Institute, 505 King Ave., Columbus, OH, 43201, USA; NC State University, Molecular Biomedical Sciences, 1060 William Moore Dr., Raleigh, NC, 27607, USA.

Forensic Science International. Genetics
|June 13, 2020
PubMed
Summary

Massively parallel sequencing (MPS) reveals significant sequence-based short tandem repeat (STR) allele variation across diverse populations. This detailed analysis enhances forensic DNA profiling accuracy and statistical power in casework.

Keywords:
IsoalleleMassively parallel sequencingPopulationShort tandem repeats

More Related Videos

Targeted Next-generation Sequencing and Bioinformatics Pipeline to Evaluate Genetic Determinants of Constitutional Disease
09:34

Targeted Next-generation Sequencing and Bioinformatics Pipeline to Evaluate Genetic Determinants of Constitutional Disease

Published on: April 4, 2018

34.5K
G2-seq: A High Throughput Sequencing-based Technique for Identifying Late Replicating Regions of the Genome
06:40

G2-seq: A High Throughput Sequencing-based Technique for Identifying Late Replicating Regions of the Genome

Published on: March 22, 2018

6.1K

Related Experiment Videos

Last Updated: Dec 18, 2025

High-throughput Identification of Gene Regulatory Sequences Using Next-generation Sequencing of Circular Chromosome Conformation Capture 4C-seq
09:06

High-throughput Identification of Gene Regulatory Sequences Using Next-generation Sequencing of Circular Chromosome Conformation Capture 4C-seq

Published on: October 5, 2018

10.7K
Targeted Next-generation Sequencing and Bioinformatics Pipeline to Evaluate Genetic Determinants of Constitutional Disease
09:34

Targeted Next-generation Sequencing and Bioinformatics Pipeline to Evaluate Genetic Determinants of Constitutional Disease

Published on: April 4, 2018

34.5K
G2-seq: A High Throughput Sequencing-based Technique for Identifying Late Replicating Regions of the Genome
06:40

G2-seq: A High Throughput Sequencing-based Technique for Identifying Late Replicating Regions of the Genome

Published on: March 22, 2018

6.1K

Area of Science:

  • Forensic Genetics
  • Population Genetics
  • Molecular Biology

Background:

  • Forensic DNA analysis is advancing with massively parallel sequencing (MPS) for higher resolution characterization.
  • Understanding sequence-based short tandem repeat (STR) allele diversity and population distribution is crucial for these advancements.

Purpose of the Study:

  • To analyze sequence-based STR allele variation and population distribution in 786 individuals from diverse US population groups.
  • To assess the impact of sequence-based genotyping on allelic diversity and forensic statistics.

Main Methods:

  • DNA amplification using PowerSeq™ Auto/Y System Prototype Kit.
  • Sequencing on an Illumina® MiSeq instrument and data analysis with Altius bioinformatics tool.
  • Capillary electrophoresis (CE) for comparative analysis and population genetics assessments (Hardy-Weinberg equilibrium, PIC, heterozygosity).

Main Results:

  • Sequence-based allelic variants found in 20 of 22 common forensic STR loci.
  • Highest sequence variation observed at D12S391.
  • Greatest increase in allelic diversity and polymorphic information content (PIC) at D3S1358 and D8S1179.

Conclusions:

  • Detailed sequence analysis of STRs reveals significant allelic variation across populations.
  • Sequence-based genotyping offers improved allelic diversity and PIC, enhancing forensic casework statistics.
  • Understanding sequence-level variation is vital for advancing forensic DNA analysis.