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Updated: Dec 18, 2025
06:52
Detection of Inflammasome Activation and Pyroptotic Cell Death in Murine Bone Marrow-derived Macrophages
Published on: May 21, 2018
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Increasing complexity of NLRP3 inflammasome regulation
Julien Moretti1,2, J Magarian Blander1,2,3,4,5
1The Jill Roberts Institute for Research in Inflammatory Bowel Disease, Weill Cornell Graduate School of Medical Science, Weill Cornell Medicine, Cornell University, New York, New York, USA.
Journal of Leukocyte Biology
|June 13, 2020
Summary
The NLRP3 inflammasome, crucial for inflammation and pyroptosis, has diverse activation triggers and complex regulation. This review details its canonical and noncanonical pathways, and recent regulatory insights.
Area of Science:
- Immunology
- Cell Biology
Background:
- Inflammasomes are multiprotein complexes sensing danger signals.
- They activate caspase-1, IL-1β secretion, and pyroptosis.
- The NLRP3 inflammasome is activated by diverse stimuli.
Purpose of the Study:
- To summarize NLRP3 inflammasome activation modes.
- To review recent insights into its regulation.
- To clarify complexities in NLRP3 inflammasome function.
Main Methods:
- Literature review of inflammasome research.
- Analysis of canonical and noncanonical NLRP3 activation pathways.
- Examination of post-translational and cellular regulatory mechanisms.
Main Results:
- NLRP3 inflammasome activation is triggered by a wide array of molecules.
- Noncanonical NLRP3 activation involves caspase-11 and intracellular LPS.
- Post-translational modifications and cellular context add complexity.
Conclusions:
- Understanding NLRP3 inflammasome activation is key to inflammatory diseases.
- Further research is needed to fully elucidate its intricate regulation.
- This review provides a comprehensive overview of current knowledge.
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