Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Microglia integrated neural spheroids enable neuroinflammatory responses and correct network dysfunction induced by alpha-synuclein mutation.

Communications biology·2026
Same author

Design, Synthesis, and Structure-Activity Optimization of Marine-Inspired Macrolactones as Nanomolar Anticancer Agents.

ACS medicinal chemistry letters·2026
Same author

Halofantrine protects photoreceptors in multiple models of retinal degeneration.

Research square·2026
Same author

Nuclear export modulates TDP-43 phase transition and cytoplasmic aggregation.

bioRxiv : the preprint server for biology·2026
Same author

Deep Learning-Guided Discovery of Dual Inhibitors of SARS-CoV-2 Entry and 3CL Protease.

Molecules (Basel, Switzerland)·2026
Same author

Generation and characterization of induced pluripotent stem cell (iPSC) lines from patients affected with Tay-Sachs and Sandhoff disease.

Stem cell research·2026

Related Experiment Video

Updated: Dec 18, 2025

A Fluorescence-based Lymphocyte Assay Suitable for High-throughput Screening of Small Molecules
08:43

A Fluorescence-based Lymphocyte Assay Suitable for High-throughput Screening of Small Molecules

Published on: March 10, 2017

10.8K

Cell-Based No-Wash Fluorescence Assays for Compound Screens Using a Fluorescence Cytometry Plate Reader.

Kirill Gorshkov1, Manisha Pradhan2, Miao Xu2

  • 1National Center for Advancing Translational Sciences, National Institutes of Health, Rockville, Maryland kirill.gorshkov@nih.gov.

The Journal of Pharmacology and Experimental Therapeutics
|June 14, 2020
PubMed
Summary

A novel wash-free fluorescence assay method enhances high-throughput screening efficiency. This laser scanning cytometry approach improves data quality and reduces processing time for cell-based assays, including disease and viral detection.

More Related Videos

A Kinetic Fluorescence-based Ca2+ Mobilization Assay to Identify G Protein-coupled Receptor Agonists, Antagonists, and Allosteric Modulators
07:41

A Kinetic Fluorescence-based Ca2+ Mobilization Assay to Identify G Protein-coupled Receptor Agonists, Antagonists, and Allosteric Modulators

Published on: February 20, 2018

9.3K
A Flow Cytometry-based Assay to Identify Compounds That Disrupt Binding of Fluorescently-labeled CXC Chemokine Ligand 12 to CXC Chemokine Receptor 4
06:56

A Flow Cytometry-based Assay to Identify Compounds That Disrupt Binding of Fluorescently-labeled CXC Chemokine Ligand 12 to CXC Chemokine Receptor 4

Published on: March 10, 2018

14.3K

Related Experiment Videos

Last Updated: Dec 18, 2025

A Fluorescence-based Lymphocyte Assay Suitable for High-throughput Screening of Small Molecules
08:43

A Fluorescence-based Lymphocyte Assay Suitable for High-throughput Screening of Small Molecules

Published on: March 10, 2017

10.8K
A Kinetic Fluorescence-based Ca2+ Mobilization Assay to Identify G Protein-coupled Receptor Agonists, Antagonists, and Allosteric Modulators
07:41

A Kinetic Fluorescence-based Ca2+ Mobilization Assay to Identify G Protein-coupled Receptor Agonists, Antagonists, and Allosteric Modulators

Published on: February 20, 2018

9.3K
A Flow Cytometry-based Assay to Identify Compounds That Disrupt Binding of Fluorescently-labeled CXC Chemokine Ligand 12 to CXC Chemokine Receptor 4
06:56

A Flow Cytometry-based Assay to Identify Compounds That Disrupt Binding of Fluorescently-labeled CXC Chemokine Ligand 12 to CXC Chemokine Receptor 4

Published on: March 10, 2018

14.3K

Area of Science:

  • Biotechnology
  • Cell Biology
  • Assay Development

Background:

  • High-throughput cell-based fluorescent imaging assays often require background signal removal, which is challenging due to equipment needs and variations from multiple wash steps.
  • Traditional methods involve extensive plate processing, leading to increased time and data file sizes.

Purpose of the Study:

  • To develop a wash-free cell-based fluorescence assay for high-throughput screening.
  • To improve assay efficiency, sensitivity, and data quality while reducing artifacts and processing time.

Main Methods:

  • Development of a wash-free cell-based fluorescence assay using a laser scanning fluorescence plate cytometer.
  • Application of the assay to Niemann-Pick disease type A and C, and detection of Zika and Dengue viral envelope proteins using the Mirrorball method.

Main Results:

  • The wash-free assay demonstrated robust screening results for lysosomal storage diseases.
  • The method was successfully applied to cholesterol detection in Niemann-Pick disease type C.
  • Preliminary results for viral envelope protein detection were obtained using the Mirrorball method.

Conclusions:

  • The Mirrorball-based method significantly improves throughput and data quality in image-based screening.
  • This versatile assay format reduces assay artifacts, increases sensitivity and efficiency, and requires less researcher intervention.