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Updated: Dec 18, 2025
Author Spotlight: Enhancing Bone Regeneration with Vascularized Artificial Cartilage Integration
Published on: July 14, 2023
Injectable and bioactive methylcellulose hydrogel carrying bone mesenchymal stem cells as a filler for critical-size
Lizhi Deng1, Yun Liu2, Liqun Yang1
1DSAPM Lab and PCFM Lab, School of Materials Science and Engineering, School of Chemistry, Sun Yat-sen University, Guangzhou, 510275, China.
Abstract:
As a soluble cellulose derivative, methylcellulose (MC), can be used to construct thermosensitive hydrogels. However, a pure MC gel is generally considered an inert material that is inadequate for cell survival. We adopted an environmentally friendly method to fabricate a nano-hydroxyapatite (nHA) hybrid MC hydrogel. Rheology results showed that the addition of nHA increased the gelation temperature. Furthermore, the live/dead assay confirmed that the addition of nHA improved the survival of bone marrow-derived mesenchymal stem cells (BMSCs) inside the gel. In addition, ARS staining indicated that the presence of nHA stimulated osteogenic differentiation. Finally, in vivo cranial defect experiments showed improved remediation efficiency when using the nHA hybrid MC hydrogel to carry BMSCs.
