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Updated: Dec 18, 2025
Simplified Reverse Genetics Method to Recover Recombinant Rotaviruses Expressing Reporter Proteins
Published on: April 17, 2020
Molecular characterization of an uncommon multigene Reassortant G1P[4] rotavirus identified in China
Yuanjun Zeng1, Biyan Zhao1, Tingdong Li1
1State Key Laboratory of Molecular Vaccinology and Molecular Diagnostics, National Institute of Diagnostics and Vaccine Development in Infectious Disease, Collaborative Innovation Center of Biological Products, School of public health, Xiamen university, Xiamen, China.
Abstract:
Rotavirus A (RVA) is the leading cause of acute gastroenteritis worldwide in both young children and animals. In this study, we analyzed the complete genome sequence of a rare G1P[4] rotavirus, which was identified from the stool samples (20130113) of an infant with diarrhea in Chongqing, China. The genotype constellation of strain 20130113 was G1-P[4]-I2-R1-C1-M1-A2-N2-T1-E1-H1. The results of both BLAST and phylogenic analysis indicated that 20130113 was likely generated by genetic reassortment between G1P[8] and G2P[4] strains that co-circulated during the season. Whether the emergence of this uncommon genotype reflects the establishment of a new RVA strain in the population requires continuous monitoring of rotavirus epidemiology.
