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Updated: Dec 18, 2025
Examining the Conformational Dynamics of Membrane Proteins in situ with Site-directed Fluorescence Labeling
Published on: May 29, 2011
BODIPY-based Fluorescent Probe for the Detection of Cysteine in Living Cells
Nannan Wang1, Xin Ji1, Han Wang1
1Key Laboratory for Special Functional Materials of Ministry of Education, School of Materials Science and Engineering, Henan University, Kaifeng, 475004, China.
Abstract:
Cysteine (Cys), as one of the important amino acids, plays a vital role in various physiological and pathological processes. Hence, it is meaningful to develop a convenient and sensitive detection method. Herein, a novel BODIPY-based fluorescent probe (BDP-DM) was developed, which had a higher selectivity for Cys than other amino acids, including homocysteine (Hcy) and glutathione (GSH). Ultimately, we concluded that the BDP-DM probe could be used to successfully detected intracellular Cys in living HeLa cells.
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