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Bulked Segregant RNA-Seq Reveals Distinct Expression Profiling in Chinese Wheat Cultivar Jimai 23 Responding to

Tong Zhu1, Liru Wu1, Huagang He2

  • 1School of Life Sciences, Yantai University, Yantai, China.

Frontiers in Genetics
|June 16, 2020
PubMed
Summary

Wheat powdery mildew resistance is crucial for crop yield. Researchers identified a single dominant gene, PmJM23, on chromosome 5DS in wheat cultivar Jimai 23, providing valuable insights into disease control mechanisms.

Keywords:
BSR-SeqDEGexpression profilingpowdery mildewwheat

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Area of Science:

  • Plant Pathology
  • Molecular Genetics
  • Agronomy

Background:

  • Wheat powdery mildew, caused by Blumeria graminis f. sp. tritici (Bgt), is a major threat to global wheat production.
  • Host resistance is the most effective control strategy, but its molecular basis in wheat remains largely unknown.

Purpose of the Study:

  • To elucidate the molecular mechanism of wheat powdery mildew resistance (Pm) in the resistant cultivar Jimai 23.
  • To identify the specific gene conferring resistance and analyze its expression patterns.

Main Methods:

  • Bulked segregant RNA-Sequencing (BSR-Seq) was employed to analyze gene expression in response to Bgt infection.
  • Single nucleotide polymorphisms (SNPs) were identified and mapped to a candidate region on chromosome 5DS.
  • Gene Ontology (GO), Clusters of Orthologous Genes (COG), and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analyses were performed on differentially expressed genes (DEGs).
  • Quantitative real-time PCR (RT-qPCR) was used to determine the temporal expression patterns of candidate genes.

Main Results:

  • A single dominant gene, tentatively named PmJM23, located in the Pm2 region of chromosome 5DS, was identified as the source of resistance in Jimai 23.
  • 3,816 consistently different SNPs were identified between resistant and susceptible parents.
  • 58 SNPs were located within the candidate region for PmJM23.
  • 3,803 DEGs were analyzed, revealing significant enrichment in specific biological pathways.
  • Six disease-related genes showed specific expression patterns during Bgt infection.

Conclusions:

  • The study identified PmJM23 as a key gene for wheat powdery mildew resistance in Jimai 23.
  • The identified DEGs and their expression patterns provide valuable genetic resources for enhancing durable resistance to Bgt in wheat breeding programs.