Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Reporter Genes

Reporter Genes

Reporter genes are a type of protein-coding gene that are often tagged to a gene of interest. Once inside a target cell, reporter genes usually produce visually identifiable characteristics like fluorescence and luminescence when expressed along with the gene of interest. Thus, reporter genes “report” the presence or absence of genes of interest in an organism, determine the gene expression pattern, or track the physical location of a DNA segment or protein in the cell.

Fluorescence and Phosphorescence: Instrumentation

Fluorescence and Phosphorescence: Instrumentation

Fluorometers and spectrofluorometers are two types of instruments used for measuring molecular fluorescence. These instruments differ in how they select excitation and emission wavelengths and the type of light sources they utilize. Fluorometers use absorption interference filters to choose excitation and emission wavelengths. The excitation source in a fluorometer is typically a low-pressure mercury vapor lamp that emits intense lines distributed throughout the ultraviolet and visible regions.

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Endoscopic iso-pathlength self-calibration for direction-resolved retrieval of tissue optical properties.

Journal of biomedical optics·2026

Same author

Correction to "Radiotherapy-Sensitized Tumor Photothermal Ablation Using γ-Polyglutamic Acid Nanogels Loaded with Polypyrrole".

Biomacromolecules·2025

Same author

Overcoming Sperm Cell Survival Challenges Cryopreserved in Nanoliter Volumes.

International journal of molecular sciences·2025

Same author

Application of PIXE for Tear Analysis: Impact of Mineral Supplementation on Iron and Magnesium Levels in Athletes.

Nutrients·2025

Same author

Combined optical-electromechanical wearable sensors for cardiac health monitoring.

Journal of biomedical optics·2025

Same author

Gold-Nanoparticles Reflectance Discriminates Benign from Malignant Salivary Gland Neoplasms.

Journal of clinical medicine·2025

Same journal

Correction to "Characterization of Cerebrovascular Changes in Mice Treated With Alcohol by Photoacoustic Imaging".

Journal of biophotonics·2026

Same journal

Defining Safe Light Intensity Limits of Near-Infrared Illumination Avoiding Skin Heating in Medical Optical Diagnostic Methods.

Journal of biophotonics·2026

Same journal

Review of the SWIR Windows to Study Osteoarthritis.

Journal of biophotonics·2026

Same journal

FTIR-ATR Spectroscopy as a Tool to Differentiate Listeria monocytogenes by Geno-Serogroups, Growth Conditions and Persistence Status.

Journal of biophotonics·2026

Same journal

Utilizing Serum Fluorescence Spectra and Machine Learning Algorithms for Efficient Diagnosis of Sheep Brucellosis.

Journal of biophotonics·2026

Same journal

Fluorescence Profiling of Water-Based Breast Tissue Homogenates Combined With Chemometric Analyses for Discrimination of Benign and Malignant Lesions.

Journal of biophotonics·2026

See all related articles

Search research articles

Related Experiment Video

Updated: Dec 18, 2025

Plasmid-derived DNA Strand Displacement Gates for Implementing Chemical Reaction Networks

Plasmid-derived DNA Strand Displacement Gates for Implementing Chemical Reaction Networks

Published on: November 25, 2015

Fluorescence for biological logic gates.

Eran A Barnoy¹, Rachela Popovtzer¹, Dror Fixler¹

¹Faculty of Engineering and the Institute of Nanotechnology and Advanced Materials, Bar Ilan University, Ramat Gan, Israel.

Journal of Biophotonics

|June 16, 2020

Summary

This summary is machine-generated.

Biological logic gates are smart nanoprobes that mimic computer logic. This review focuses on using fluorescence signals to create these advanced diagnostic tools for detecting complex disease conditions.

Keywords:

FLIM biological logic gates fluorescence molecular logic gates reactive probes smart probes

More Related Videos

Bioluminescent Optogenetics 2.0: Harnessing Bioluminescence to Activate Photosensory Proteins In Vitro and In Vivo

Bioluminescent Optogenetics 2.0: Harnessing Bioluminescence to Activate Photosensory Proteins In Vitro and In Vivo

Published on: August 4, 2021

Gene Digital Circuits Based on CRISPR-Cas Systems and Anti-CRISPR Proteins

Gene Digital Circuits Based on CRISPR-Cas Systems and Anti-CRISPR Proteins

Published on: October 18, 2022

Related Experiment Videos

Last Updated: Dec 18, 2025

Plasmid-derived DNA Strand Displacement Gates for Implementing Chemical Reaction Networks

Plasmid-derived DNA Strand Displacement Gates for Implementing Chemical Reaction Networks

Published on: November 25, 2015

Bioluminescent Optogenetics 2.0: Harnessing Bioluminescence to Activate Photosensory Proteins In Vitro and In Vivo

Bioluminescent Optogenetics 2.0: Harnessing Bioluminescence to Activate Photosensory Proteins In Vitro and In Vivo

Published on: August 4, 2021

Gene Digital Circuits Based on CRISPR-Cas Systems and Anti-CRISPR Proteins

Gene Digital Circuits Based on CRISPR-Cas Systems and Anti-CRISPR Proteins

Published on: October 18, 2022

Area of Science:

Biomedical Engineering
Nanotechnology
Molecular Diagnostics

Background:

Biological logic gates are sophisticated nanoprobes designed to respond to specific biological conditions.
These nanodevices offer potential for advanced medical diagnostics by integrating multiple biological parameter detection.

Purpose of the Study:

To review the development and application of biological logic gates for medical applications.
To focus on logic operations actuated through fluorescent signals for disease detection.

Main Methods:

Exploration of various stimuli-responsive nanodevices, including fluorescent probes and DNA nanomachines.
Analysis of logic operations implemented using fluorescence intensity and fluorescence lifetime.

Main Results:

Numerous nanodevices capable of performing logic operations have been developed.
Fluorescence-based methods are a viable approach for implementing biological logic gates.

Conclusions:

Biological logic gates represent a promising frontier in modern medicine.
Further research into fluorescence-actuated logic operations can enhance disease detection capabilities.