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Updated: Dec 18, 2025
Flow Cytometric Characterization of Murine B Cell Development
Published on: January 22, 2021
Flow-cytometry as an auxiliary in the diagnosis of primary humoral immunodeficiencies
Leopoldo Santos-Argumedo1, Laura Berrón-Ruiz1, Gabriela López-Herrera1
1Instituto Politécnico Nacional, Center of Research and Advanced Studies, Department of Molecular Biomedicine, Mexico City, Mexico.
Background:
Antibody deficiencies encompass a wide spectrum of pathologies and constitute approximately 50 % of primary immunodeficiencies; with cytometry, it is possible to evaluate the immune status rapidly, effectively and at low cost.
Objective:
To assess, by means of flow cytometry, the cells of patients with three types of primary humoral immunodeficiencies.
Method:
Using flow cytometry, blood samples from patients and healthy subjects were analyzed with different monoclonal antibodies.
Results:
Using various stains, a severe decrease in B lymphocytes was shown in patients with X-linked agammaglobulinemia, as well as a lack of CD154 expression in patients with hyper-immunoglobulin M syndrome, and heterogeneity of B lymphocyte subpopulations in patients with common variable immunodeficiency.
Conclusion:
Flow cytometry enables early diagnosis of primary immunodeficiencies with a high level of confidence and, in many cases, identification of the genes involved.
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