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Updated: Dec 18, 2025

Sample Preparation Strategies for Mass Spectrometry Imaging of 3D Cell Culture Models
Published on: December 5, 2014
A Versatile Platform for Mass Spectrometry Imaging of Arbitrary Spatial Patterns
Kenneth P Garrard1,2,3, Måns Ekelöf1, Sitora Khodjaniyazova1
1FTMS Laboratory for Human Health Research, Department of Chemistry, North Carolina State University, Raleigh, North Carolina 27695, United States.
Abstract:
A vision-system driven platform, RastirX, has been constructed for mass spectrometry imaging (MSI) of arbitrary two-dimensional patterns. The user identifies a region of interest (ROI) by drawing on a live video image of the sample with the computer mouse. Motion commands are automatically generated to move the sample to acquire scan data for the pixels in the ROI. Synchronization of sample stage motion with laser firing and mass spectrometer (MS) scan acquisition is fully automated. RastirX saves a co-registered optical image and the scan location information needed to convert raw MS data into imzML format. Imaging an arbitrarily shaped ROI instead of the minimal enclosing rectangle reduces contamination from off-sample material and significantly reduces acquisition time.
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