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Hydrogen peroxide-response nanoprobe for CD44-targeted circulating tumor cell detection and H2O2 analysis
Chunting Li1, Jiexuan Wang2, Xinmiao Lu1
1Department of Nuclear Medicine, Ruijin Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, 200025, People's Republic of China.
Biomaterials
|June 17, 2020
Summary
This study introduces novel H2O2-responsive nanoprobes that target CD44 proteins on pancreatic cancer cells. These nanoprobes improve circulating tumor cell detection and quantification, aiding in cancer diagnosis and prognosis.
Area of Science:
- Biomedical Engineering
- Nanotechnology
- Oncology
Background:
- Circulating tumor cells (CTCs) are key indicators of metastasis, crucial for cancer diagnosis, staging, and prognosis.
- Detecting and analyzing CTCs is challenging due to their low abundance and interference from other blood cells.
Purpose of the Study:
- To develop and evaluate H2O2-responsive nanoprobes with CD44-targeting capabilities for enhanced CTC detection.
- To reduce interference from blood cells and improve the accuracy of CTC analysis.
Main Methods:
- Synthesized amphiphilic nanoprobes by combining a hydrophobic H2O2-responsive naphthalimide-borate fluorophore with hydrophilic hyaluronic acid.
- Utilized CD44-overexpressing pancreatic cancer cells to assess the nanoprobes' specificity and efficacy.
- Evaluated the nanoprobes' ability to recognize cancer cells and semi-quantify hydrogen peroxide (H2O2) levels.
Main Results:
- The developed nanoprobes specifically recognized pancreatic cancer cells expressing CD44 proteins.
- The nanoprobes effectively reduced interference from white blood cells in peripheral blood samples.
- The nanoprobes demonstrated capability in semi-quantifying intracellular H2O2 content within CTCs.
Conclusions:
- The H2O2-responsive, CD44-targeted nanoprobes offer a promising tool for improving CTC detection and analysis.
- These nanoprobes can aid in cancer clinical evaluation and therapy monitoring by providing accurate CTC and H2O2 quantification.
