Related Experiment Video

Updated: Dec 18, 2025

A Macrophage Reporter Cell Assay to Examine Toll-Like Receptor-Mediated NF-kB/AP-1 Signaling on Adsorbed Protein Layers on Polymeric Surfaces
07:55

A Macrophage Reporter Cell Assay to Examine Toll-Like Receptor-Mediated NF-kB/AP-1 Signaling on Adsorbed Protein Layers on Polymeric Surfaces

Published on: January 7, 2020

7.7K

Cutting Edge: TNF Is Essential for Mycobacteria-Induced MINCLE Expression, Macrophage Activation, and Th17

Judith Schick1, Johanna Schäfer1, Christian Alexander2

  • 1Institut für Klinische Mikrobiologie, Immunologie und Hygiene, Universitätsklinikum Erlangen, Friedrich-Alexander Universität Erlangen-Nürnberg, 91054 Erlangen, Germany.

Journal of Immunology (Baltimore, Md. : 1950)
|June 17, 2020
PubMed

Insights

Tumor necrosis factor (TNF) blockade upregulates C-type lectin receptors (CLRs) like MINCLE, MCL, and DECTIN-2 in macrophages, impacting immune responses and vaccine efficacy.

Area of Science:

  • Immunology
  • Molecular Biology
  • Microbiology

Background:

  • Tumor necrosis factor (TNF) blockade is effective for autoimmune diseases but increases infection susceptibility.
  • C-type lectin receptors (CLRs), including MINCLE, MCL, and DECTIN-2, are crucial for sensing microbial components on myeloid cells.

Purpose of the Study:

  • To investigate the role of TNF in regulating CLR expression on macrophages.
  • To determine the impact of TNF on immune responses to mycobacterial antigens and vaccine adjuvants.

Main Methods:

  • Macrophage cultures treated with TNF and/or MINCLE ligands.
  • Stimulation with *Mycobacterium bovis* bacillus Calmette-Guérin (BCG).
  • Analysis of CLR expression and cytokine secretion.
  • Assessment of Th17 responses in TNF-deficient mice and mice treated with etanercept (TNF blockade).

Main Results:

  • TNF administration upregulated MINCLE, MCL, and DECTIN-2 expression in macrophages.
  • TNF signaling via TNFR1 p55 was essential for CLR upregulation and cytokine secretion.
  • TNF blockade or deficiency abrogated Th17 responses to a MINCLE-dependent adjuvant.

Conclusions:

  • TNF signaling is critical for the expression of DECTIN-2 family CLRs.
  • Interference with TNF antagonizes CLR expression, potentially impairing vaccine responses and increasing infection risk.

More Related Videos

Fluorescence Assays for the Study of Mycobacterium tuberculosis Interaction with the Immune Receptor SLAMF107:42

Fluorescence Assays for the Study of Mycobacterium tuberculosis Interaction with the Immune Receptor SLAMF1

Published on: February 28, 2025

818
Isolation Protocol of Mouse Monocyte-derived Dendritic Cells and Their Subsequent In Vitro Activation with Tumor Immune Complexes11:48

Isolation Protocol of Mouse Monocyte-derived Dendritic Cells and Their Subsequent In Vitro Activation with Tumor Immune Complexes

Published on: May 31, 2018

11.9K

Related Concept Videos

T Cell Types and Functions01:24

T Cell Types and Functions

When T cells with CD4 markers are activated, they give rise to two types of effector cells: helper T cells and regulatory T cells. Meanwhile, T cells with CD8 markers differentiate into effector cytotoxic T cells. The differentiation of CD4 T cells into helper T cell subsets, such as Th1, Th2, and Th17 cells, is dependent on the antigen type, antigen-presenting cell, and regulatory cytokines.
Th1 cells stimulate dendritic cells to express necessary co-stimulatory molecules on their surfaces for...
2.0K
NF-κB-dependent Signaling Pathway02:26

NF-κB-dependent Signaling Pathway

The transcription factor NF-κB was discovered in 1986 in the lab of Nobel laureate Professor David Baltimore, for its interaction with the immunoglobulin light chain enhancer in B-cells. After more than three decades of study, it is now evident that NF-κB regulates the expression of over 100 genes. Most of these genes play an essential role in the innate and adaptive immune responses as well as the inflammatory responses of animals.
NF-κB-dependent Signaling Mechanism
The...
9.7K
Defense Against Bacterial Pathogens01:31

Defense Against Bacterial Pathogens

The human immune system is a complex network of cells, tissues, and organs that work together to defend the body against bacterial infections. It consists of various immune cells, each playing a specific role in the defense mechanism.
Phagocytes
Phagocytes are the frontline soldiers of the immune system. They include neutrophils and macrophages. Neutrophils are the most abundant type of white blood cell and are quickly mobilized to the site of infection. Macrophages are larger cells that patrol...
2.5K
T Cell Activation and Clonal Selection01:22

T Cell Activation and Clonal Selection

T cells are integral to our adaptive immune system, recognizing and effectively responding to foreign antigens. T cell activation and clonal selection are pivotal in orchestrating this immune response. This article elucidates these mechanisms, detailing the roles of cluster of differentiation (CD) markers, major histocompatibility complex (MHC) molecules, costimulatory signals, and the process of clonal selection.
Naive T cells that have not yet encountered an antigen express two primary CD...
14.3K