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A lipidome atlas in MS-DIAL 4
Hiroshi Tsugawa1,2, Kazutaka Ikeda3,4, Mikiko Takahashi5
1RIKEN Center for Integrative Medical Sciences, Yokohama, Japan. hiroshi.tsugawa@riken.jp.
Abstract:
We present Mass Spectrometry-Data Independent Analysis software version 4 (MS-DIAL 4), a comprehensive lipidome atlas with retention time, collision cross-section and tandem mass spectrometry information. We formulated mass spectral fragmentations of lipids across 117 lipid subclasses and included ion mobility tandem mass spectrometry. Using human, murine, algal and plant biological samples, we annotated and semiquantified 8,051 lipids using MS-DIAL 4 with a 1-2% estimated false discovery rate. MS-DIAL 4 helps standardize lipidomics data and discover lipid pathways.
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