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Updated: Dec 18, 2025
In Vivo Inhibition of MicroRNA to Decrease Tumor Growth in Mice
Published on: August 23, 2019
MiR-345-5p inhibits tumorigenesis of papillary thyroid carcinoma by targeting SETD7
Ming Zhao1, Kejing Wang1, Jinbiao Shang1
1Department of Head and Neck Surgery, Zhejiang Cancer Hospital, Zhejiang, China.
Introduction:
This study aimed to explore the effects of miR-345-5p on papillary thyroid carcinoma (PTC) and uncover the possible mechanisms.
Material And Methods:
MiR-345-5p and SETD7 mRNA levels were analyzed by quantitative real-time PCR and SETD7 protein level was measured by Western blot. The viability, colony formation ability and apoptosis of PTC cells were measured with CCK-8, soft agar colony formation and flow cytometry assay, respectively. Luciferase reporter assay was used to identify miR-345-5p's target.
Results:
Compared to neighboring normal tissues, there was lower miR-345-5p expression and higher SETD7 expression in PTC tissues. Moreover, Spearman's correlation analysis indicated that there was a negative correlation between miR-345-5p and SETD7 expression in PTC tissues. MiR-345-5p mimics inhibited the viability and colony formation of TPC1 and B-CPAP cells and promoted apoptosis, whereas anti-miR-345-5p promoted PTC cell proliferation and inhibited apoptosis. SETD7 was confirmed to be a direct target of miR-345-5p through target scan analysis and luciferase reporter assay. Additionally, overexpression of SETD7 promoted the viability and colony formation of TPC1 and B-CPAP cells and inhibited apoptosis, whereas downregulation of SETD7 by shRNAs had opposite effects on PTC cells. Furthermore, overexpression of SETD7 attenuated the miR-345-5p induced anti-tumor effects on PTC cells.
Conclusions:
MiR-345-5p exhibited suppressive effects on PTC via targeting SETD7.
Insights
MicroRNA-345-5p suppresses papillary thyroid carcinoma (PTC) by targeting SETD7. Lower miR-345-5p and higher SETD7 expression in PTC tissues indicate this tumor-suppressive role.
Area of Science:
- Molecular Biology
- Oncology
- Gene Regulation
Background:
- Papillary thyroid carcinoma (PTC) is a prevalent endocrine malignancy.
- Understanding the molecular mechanisms underlying PTC progression is crucial for developing targeted therapies.
Purpose of the Study:
- To investigate the role of microRNA-345-5p (miR-345-5p) in papillary thyroid carcinoma (PTC).
- To elucidate the underlying molecular mechanisms, including the identification of miR-345-5p targets.
Main Methods:
- Quantitative real-time PCR and Western blot were used to analyze miR-345-5p and SETD7 expression.
- Cell viability, colony formation, and apoptosis assays were performed.
- Luciferase reporter assays confirmed the direct targeting of SETD7 by miR-345-5p.
Main Results:
- MiR-345-5p was downregulated, while SETD7 was upregulated in PTC tissues, showing a negative correlation.
- MiR-345-5p mimics inhibited PTC cell proliferation and induced apoptosis; anti-miR-345-5p had opposite effects.
- SETD7 acts as a direct target of miR-345-5p, and its modulation affects PTC cell behavior.
Conclusions:
- MiR-345-5p functions as a tumor suppressor in PTC.
- The suppressive effects of miR-345-5p are mediated through its direct targeting of SETD7.
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