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Dynamic single-cell intracellular pH sensing using a SERS-active nanopipette.

Jing Guo1, Alberto Sesena Rubfiaro, Yanhao Lai

  • 1Department of Physics, Florida International University, 11200 SW 8th St., Miami, FL 33199, USA. jinhe@fiu.edu.

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|June 17, 2020
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Summary

This study presents a novel plasmonic nanopipette for long-term, minimally invasive intracellular analysis. The device enables sensitive pH sensing in living cells, offering insights into cellular responses to environmental changes.

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Area of Science:

  • Nanotechnology
  • Biomedical Engineering
  • Analytical Chemistry

Background:

  • Glass nanopipettes are valuable tools for single-cell studies.
  • Plasmonic nanopipettes enhance single-cell analysis using surface-enhanced Raman spectroscopy (SERS).

Purpose of the Study:

  • To develop a SERS-active nanopipette for long-term, reliable intracellular analysis of living cells with minimal damage.
  • To demonstrate the nanopipette's capability for intracellular pH sensing.

Main Methods:

  • Optimized nanopipette geometry and gold nanoparticle (AuNP) layer density.
  • Modified AuNP layer with 4-mercaptobenzoic acid for pH sensitivity.
  • Utilized SERS for intracellular pH measurements in HeLa and fibroblast cells.

Main Results:

  • Achieved a pH sensing range of 6.0-8.0 with a response time under 5 seconds and a maximum sensitivity of 0.2 pH units.
  • Monitored intracellular pH changes in response to extracellular pH variations.
  • Observed that HeLa cancer cells exhibit greater resilience to extracellular pH changes.

Conclusions:

  • The developed SERS-active nanopipette enables precise, long-term intracellular pH monitoring in living cells.
  • This technology can be extended for the detection of other intracellular biomarkers.
  • The findings provide a new tool for understanding cellular behavior and disease states.