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Updated: Dec 18, 2025
Characterization and Isolation of Mouse Primary Microglia by Density Gradient Centrifugation
Published on: February 16, 2018
Retraction Note: Selected TLR7/8 agonist and type I interferon (IFN‑α) cooperatively redefine the microglia
Mst Reshma Khatun1, Sarder Arifuzzaman2
1Department of Biomedical Science, Ajou University, Suwon, Gyeonggi-do, 16499, Republic of Korea.
Abstract:
Since publication, it has become apparent that there are major errors in the qRT-PCR data presented.
Insights
Major errors were found in the quantitative real-time polymerase chain reaction (qRT-PCR) data after publication. This necessitates a critical re-evaluation of the study's findings and conclusions.
Area of Science:
- Molecular Biology
- Genetics
- Biochemistry
Background:
- Quantitative real-time polymerase chain reaction (qRT-PCR) is a widely used technique for gene expression analysis.
- Accurate qRT-PCR data is crucial for reliable scientific conclusions.
Purpose of the Study:
- To address and correct significant errors identified in previously published qRT-PCR data.
- To ensure the integrity and reproducibility of research findings.
Main Methods:
- Re-analysis of existing qRT-PCR data.
- Identification and quantification of errors in data presentation.
- Application of corrected data for re-evaluation.
Main Results:
- Major discrepancies and errors were identified in the original qRT-PCR data.
- The extent of these errors impacts the validity of the initial findings.
Conclusions:
- The previously reported findings based on the erroneous qRT-PCR data are unreliable.
- A thorough review and correction of the data are essential for scientific accuracy.
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