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Quantification and Pathogenicity of Candida albicans in Denture-Wearing and Nondenture-Wearing Elderly
Boy M Bachtiar1, Turmidzi Fath2, Retno Widowati2
1Department of Oral Biology and Oral Science Research Center, Faculty of Dentistry, Universitas Indonesia, Jakarta, Indonesia.
Objective:
The primary purpose of this study was to evaluate and compare the microbial loads and pathogenicity traits of oral Candida albicans in denture-wearing (DW; n = 15) and nondenture-wearing (NDW; n = 15) elderly persons.
Materials And Methods:
The fungal counts of the saliva, tongue dorsa, and prosthesis-fitting surfaces of the participants were assessed using real-time polymerase chain reaction to compare the quantity and expression of selected C. albicans biofilm-associated genes (ALS3, HWP1, and YWP1).
Statistical Analysis:
The obtained data were analyzed by one-way analysis of variance, followed by Bartlett's test. When appropriate, the Student's t-test was also used; a value of p < 0.05 was considered statistically significant.
Results:
In both groups, the count of C. albicans was found to be significantly higher in saliva than in other oral samples. The expression of the hypha-specific genes (ALS3 and HWP1) in the tongue dorsa was higher in the DW group (p < 0.05), whereas the transcription level of the yeast-specific gene (YWP1) was significantly higher in the NDW group.
Conclusion:
Both tongue dorsa and dentures appear to be sharing factors that are important for C. albicans biofilm growth in abiotic and biotic oral surfaces of the elderly.
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