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Updated: Dec 18, 2025

Mapping Bacterial Functional Networks and Pathways in Escherichia Coli using Synthetic Genetic Arrays
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Mapping Bacterial Functional Networks and Pathways in Escherichia Coli using Synthetic Genetic Arrays

Published on: November 12, 2012

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Gene interaction network approach to elucidate the multidrug resistance mechanisms in the pathogenic bacterial strain

Sravan K Miryala1, Anand Anbarasu1, Sudha Ramaiah1

  • 1Medical and Biological Computing Laboratory, Department of Bio-Sciences, School of Biosciences and Technology, Vellore Institute of Technology, Vellore, India.

Journal of Cellular Physiology
|June 17, 2020
PubMed

Insights

Proteus mirabilis causes urinary tract infections and multidrug resistance. This study identifies novel drug targets, including rpoB and tufB genes, to combat antibiotic resistance in P. mirabilis.

Area of Science:

  • Microbiology
  • Genomics
  • Drug Discovery

Background:

  • Proteus mirabilis is a common cause of urinary tract infections.
  • Multidrug resistance in P. mirabilis complicates treatment and necessitates new therapeutic strategies.

Purpose of the Study:

  • To analyze the evolutionary relationships of P. mirabilis strains.
  • To understand the molecular basis of antibiotic resistance (AR) in P. mirabilis.
  • To identify potential new drug targets for combating multidrug-resistant P. mirabilis.

Main Methods:

  • Analysis of evolutionary relationships of 29 P. mirabilis strains from NCBI-Genome database.
  • Gene network analysis of antimicrobial resistance genes, enriched pathways, and Gene Ontology terms.
  • Identification of potential drug targets based on their role in vital bacterial functions.

Main Results:

  • Identified key genes such as rpoB, tufB, rpsl, fusA, and rpoA as potential drug targets.
  • Elucidated the molecular underpinnings of antibiotic resistance through gene network analysis.
  • Provided insights into the evolutionary landscape of P. mirabilis.

Conclusions:

  • The identified genes (rpoB, tufB, rpsl, fusA, rpoA) represent promising targets for developing novel therapies against multidrug-resistant P. mirabilis.
  • This research aids in the development of new strategies to overcome antibiotic resistance in P. mirabilis infections.
Keywords:
Proteus mirabilisclustering analysisfunctional enrichment analysisgene ontologytwo-component system

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