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Fixation and Sectioning

Fixation and Sectioning

Two basic types of preparation are used to visualize specimens with a light microscope: wet mounts and fixed specimens.
The simplest type of preparation is the wet mount, in which the specimen is placed in a drop of liquid on the slide. A liquid specimen can be directly deposited on the slide using a dropper. Solid specimens, such as skin scraping, can be placed on the slide before adding a drop of liquid to prepare the wet mount. Sometimes the liquid is simply water, but stains are often added...

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Related Experiment Video

Updated: Dec 18, 2025

Author Spotlight: Decoding Mitochondrial Aging

Author Spotlight: Decoding Mitochondrial Aging

Published on: June 30, 2023

[Comparison Between Different Mitochondrial Staining Methods in Cell and Tissue Samples].

Dan Long¹, Yan-Ni Zhou¹, Li Feng¹

¹Key Laboratory of Transplant Engineering and Immunology of the Ministry of Health, West China Hospital, Sichuan University, Chengdu 610041, China.

Sichuan Da Xue Xue Bao. Yi Xue Ban = Journal of Sichuan University. Medical Science Edition

|June 17, 2020

Summary

This summary is machine-generated.

For cell imaging, anti-Grp75 antibody provides clearer mitochondrial visualization than MitoTracker Deep Red probes. For tissue samples, MitoTracker Deep Red probes offer superior staining effects.

Keywords:

Grp75 MitoTracker Deep Red Mitochondria Probe

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Multicolor Flow Cytometry-based Quantification of Mitochondria and Lysosomes in T Cells

Multicolor Flow Cytometry-based Quantification of Mitochondria and Lysosomes in T Cells

Published on: January 9, 2019

Area of Science:

Cell Biology
Histology
Biotechnology

Background:

Accurate visualization of mitochondria is crucial for understanding cellular function and disease.
Mitochondrial staining techniques, including fluorescent probes and antibody-based methods, are widely used.
Comparing the efficacy of different staining methods in various biological samples is essential for optimizing research outcomes.

Purpose of the Study:

To compare the effectiveness of MitoTracker Deep Red fluorescent probes versus anti-Grp75 antibody for mitochondria staining.
To evaluate staining performance in both cell lines (HepG2, HeLa) and human liver tissue samples.
To determine the optimal staining method for different sample types in microscopy.

Main Methods:

HepG2 and HeLa cells were fixed and stained with MitoTracker Deep Red or anti-Grp75 antibody.
Human healthy liver tissue samples were similarly stained with the same probes and antibody.
Stained samples were analyzed using confocal microscopy to assess staining quality and specificity.

Main Results:

MitoTracker Deep Red exhibited non-specific staining and produced obscure mitochondrial images in HeLa cells.
Anti-Grp75 antibody demonstrated clear tubular and punctate mitochondrial distribution in cell samples.
MitoTracker Deep Red showed more specific and effective staining in liver tissue samples compared to the antibody.

Conclusions:

Anti-Grp75 antibody staining is more suitable for visualizing mitochondria in cell samples.
MitoTracker Deep Red probes are more appropriate for staining mitochondria in tissue samples.
The choice of staining method should be tailored to the specific cell or tissue type being investigated.