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Updated: Jul 26, 2026

A Guide to Structured Illumination TIRF Microscopy at High Speed with Multiple Colors
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Tuning the resolution and depth of field of a lens using an adjustable ring beam illumination
Abstract:
A pair of axicons with an adjustable separation between them is used to generate a variable diameter ring beam with high efficiency. This beam illuminates a lens to produce quasi-diffraction-free beams with a tunable spot size and depth of field. We studied the generated beam characteristics while changing either the ring diameter or its thickness. Such a scheme has applications in adjustable imaging, including nondiffracting beam microscopy, material processing with an irradiance above a certain threshold value, and particle trapping/manipulation.
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